PXD041577 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Plasma proteomics of BACE1, 2, and double KO mice |
| Description | The beta-secretase BACE1 is a central drug target for Alzheimer’s disease. Clinically tested, BACE1-directed inhibitors also block the homologous protease BACE2. Yet, little is known about physiological BACE2 substrates and functions in vivo. Here, we performed glycoprotein enrichment and subsequent discovery proteomics to identify substrates of the protease BACE2 in plasma of mice. Therefore, we analysed plasma from BACE2 KO, BACE1/2 double KO and WT controls, as well as BACE1 KO with a separate WT control. Inactivation of BACE2, but not BACE1, inhibited shedding of VEGFR3/FLT4. Thus, sVEGFR3 represents a pharmacodynamic plasma marker for BACE2 activity in vivo. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-06-27 |
| AnnouncementXML | Submission_2024-06-27_09:43:07.981.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Stephan Mueller |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-04-17 03:17:34 | ID requested | |
| ⏵ 1 | 2024-06-27 09:43:09 | announced | |
| 2 | 2024-10-22 06:47:47 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: BACE2, glycocapture, beta secretase, plasma |
Contact List
| Stefan F. Lichtenthaler |
|---|
| contact affiliation | DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany |
| contact email | stefan.lichtenthaler@dzne.de |
| lab head | |
| Stephan Mueller |
|---|
| contact affiliation | DZNE Munich Neuroproteomics |
| contact email | stephan.mueller@dzne.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD041577 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041577
- Label: PRIDE project
- Name: Plasma proteomics of BACE1, 2, and double KO mice
