PXD041550-2

PXD041550 is an original dataset announced via ProteomeXchange.

Title	Identification of DYRK3 interaction partners by immunoprecipitation-MS and TurboID
Description	Proteins that interact with overexpressed GFP-DYRK3 were pulled down for identification by LC-MS/MS. Interaction strength and likelihood were calculated by comparison to pulldown of GFP alone. Data were compared to Turbo-ID proximity labelling data for DYRK3 in HeLa cells to identify consistently associated proteins. These included many secretory proteins. Further experiments established that DYRK3 phosphorylates SEC16A to regulate the liquid-liquid phase separation of ER exit sites.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:50:15.786.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katrina Meyer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-14 11:44:50	ID requested
1	2023-06-23 06:08:59	announced
⏵ 2	2024-10-22 05:50:16	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: HEK293T cells, Hela cells, TurboID, DYRK3, phosphorylation, immunoprecipitation, kinase

Lucas Pelkmans
contact affiliation	Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
contact email	lucas.pelkmans@mls.uzh.ch
lab head
Katrina Meyer
contact affiliation	MPI Molecular Genetics
contact email	katrina.meyer@uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041550

PRIDE project URI

• PRIDE
  1. PXD041550
     1. Label: PRIDE project
     2. Name: Identification of DYRK3 interaction partners by immunoprecipitation-MS and TurboID