PXD041544 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics of expanded resting and activated human MAIT (mucosal associated invariant T cells) |
Description | Mucosal associated invariant T (MAIT) cells are an abundant population of innate T cells that recognize bacterial ligands and play a key role in host protection against bacterial and viral pathogens. Upon activation, MAIT cells undergo proliferative expansion and increase their production of effector molecules such as cytokines. In this study, we found that mRNA and protein the abundance of the key metabolism regulator and transcription factor MYC was increased in stimulated MAIT cells. Using quantitative mass spectrometry, we identified the activation of two MYC controlled metabolic pathways, amino acid transport and glycolysis, both of which were necessary for MAIT cell proliferation. Finally, we showed that MAIT cells isolated from people with obesity showed decreased MYC mRNA abundance upon activation, which was associated with defective MAIT cell proliferation and functional responses. Collectively, our data uncovers the importance of MYC-regulated metabolism for MAIT cell proliferation and provides additional insight into the molecular basis for the functional defects of MAIT cells in obesity. |
HostingRepository | PRIDE |
AnnounceDate | 2023-04-20 |
AnnouncementXML | Submission_2023-04-20_03:09:22.584.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AndrewHowden |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-14 07:45:01 | ID requested | |
⏵ 1 | 2023-04-20 03:09:22 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: T-lymphoctes, T-cells, Myc, proteomics,MAIT, human |
Contact List
Dr AndrewHogan |
contact affiliation | Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co Kildare. Ireland. |
contact email | andrew.e.hogan@mu.ie |
lab head | |
AndrewHowden |
contact affiliation | University of Dundee |
contact email | a.howden@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041544
- Label: PRIDE project
- Name: Quantitative proteomics of expanded resting and activated human MAIT (mucosal associated invariant T cells)