PXD041470 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comprehensive proteome profiling of a Xanthomonas campestris pv campestris B100 culture grown in a minimal medium with a specific focus on nutrient consumption and xanthan biosynthesis |
Description | Xanthan is the most used polysaccharide of bacterial origin. There are many industrial applications for xanthan, especially in food products. The organism Xanthomonas campestris is commonly used for xanthan fermentation, but little is known about the xanthan synthesis mechanism on the proteome level. The study used a label-free LC-MS/MS method to investigate the protein shifts during a typical xanthan fermentation of 72 hours. Two thousand four hundred sixteen proteins were identified, representing 54.75 % of the 4413 entries in the reference database. One thousand seven hundred forty-nine proteins were measured in a quantifiable quality. The quality of the proteome data was examined by the abundance changes of the ribosomal proteins according to the growth phases. The phenotypic changes during the cultivation, especially processes known to be influencing or close related to xanthan production, were investigated by proteome analysis. These processes were nitrogen metabolism, sugar nucleotide metabolism, and xanthan synthesis. This study shows the abundance of changes in central metabolites during a xanthan fermentation of Xcc. The presented research provides crucial information for further biotechnological studies to improve xanthan production. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-24 |
AnnouncementXML | Submission_2024-05-24_02:16:25.593.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ben Struck |
SpeciesList | scientific name: Xanthomonas campestris pv. campestris str. B100; NCBI TaxID: 509169; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-11 07:59:14 | ID requested | |
⏵ 1 | 2024-05-24 02:16:26 | announced | |
Publication List
10.3390/proteomes12020012; |
Struck B, Wiersma SJ, Ortseifen V, P, ΓΌ, hler A, Niehaus K, pv. Campestris B100 Culture Grown in Minimal Medium with a Specific Focus on Nutrient Consumption and Xanthan Biosynthesis. Proteomes, 12(2):(2024) [pubmed] |
Keyword List
submitter keyword: Quantitative proteomics, Xanthan fermentation, Time course, Xanthomonas campestris pv. campestris B100,Bacterial proteome, LC-MS/MS |
Contact List
Prof. Dr. Karsten Niehaus |
contact affiliation | Bielefeld University, Center for Biotechnology CeBiTec, |
contact email | kniehaus@cebitec.uni-bielefeld.de |
lab head | |
Ben Struck |
contact affiliation | University Bielefeld |
contact email | bstruck@cebitec.uni-bielefeld.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041470
- Label: PRIDE project
- Name: Comprehensive proteome profiling of a Xanthomonas campestris pv campestris B100 culture grown in a minimal medium with a specific focus on nutrient consumption and xanthan biosynthesis