PXD041463 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic shift in mouse embryonic fibroblasts Pfa1 during erastin-induced ferroptosis |
| Description | This report provides data on ferroptosis induced proteomic response in immortalized mouse embryonic fibroblasts which are 4-OH-TAM-inducible Gpx4−/− (referred to as Pfa1 cells). Pfa1 cells are a valuable, widespread and well characterized model for ferroptosis research. In the first part of this batch, we used Erastin to induce ferroptosis and collected samples at 0h, 24h and 48h after treatment. Untreated cells were used as a control. In the second part, we tested Liproxtatin-1 ferroptosis inhibitor on Tamoxifen-induced Pfa1 cells after 0h, 24h, 48h. Tamoxifen-induced ferroptosis activation of these cells are in PRIDE PXD040094. Pfa1 cells (a kind gift from Marcus Conrad, Munich) were cultured in RPMI-1640 media (2 g/L glucose, 10% FBS, 2 mM Gibco GlutaMAX Supplement, 1% pen/strep) at 37C with 5% CO2 in a humidified incubator. Cells (300k) were seeded on Corning 100 mm tissue-culture treated culture dishes and incubated overnight. On the next day Pfa1 cells were treated with or without 0.5 µM erastin (E7781, Sigma-Aldrich). For experiment on ferroptosis inhibition Pfa1 cells were treated with or without 1 µM Tamoxifen and 0.5 µM Liproxtatin-1 simultaneously. |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-11-14 |
| AnnouncementXML | Submission_2023-11-14_06:40:27.904.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD041463 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Alexey Nesterenko |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-04-11 04:06:14 | ID requested | |
| 1 | 2023-07-11 00:47:55 | announced | |
| 2 | 2023-07-24 02:37:44 | announced | 2023-07-24: Updated project metadata. |
| ⏵ 3 | 2023-11-14 06:40:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: proteomics,ferroptosis, mouse embryonic fibroblasts, erastin, glutathione peroxidase 4, Liproxtatin-1, Pfa1, cell death |
Contact List
| Vsevolod V. Belousov |
|---|
| contact affiliation | Institute of Fundamental Neuroscience, Federal Center of Brain Research and Neurotechnologies, FMBA 117513 Moscow Russia |
| contact email | vsevolod.belousov@gmail.com |
| lab head | |
| Alexey Nesterenko |
|---|
| contact affiliation | Federal Center of Brain Research and Neurotechnologies |
| contact email | comcon1@protonmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD041463 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041463
- Label: PRIDE project
- Name: Proteomic shift in mouse embryonic fibroblasts Pfa1 during erastin-induced ferroptosis
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