PXD041404-2

PXD041404 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early MSCs osteogenic differentiation
Description	The intriThe intricate balance between MSCs differentiation to osteoblasts or adipocytes is finely regulated. To explore novel participating molecules, we screened for early-stage osteogenesis- or adipogenesis-based MSCs protein expression profile using TMT-based quantitative proteomic analysis. Protein annotation, hierarchical clustering, functional stratification, and protein-protein association assessments were performed. Moreover, two upregulated proteins, namely, FBLN2 and NPR3, were validated to participate in the osteogenic differentiation process of MSCs. Subsequently, we independently downregulated FBLN2 and NPR3 during 7 days of osteogenic differentiation, and conducted quantitative proteomics analysis to assess the differential protein regulation between knockdown and control cells. Based on gene ontology (GO) and network analyses, FBLN2 deficiency induced functional alterations associated with biological regulation and stimulus response, whereas, NPR3 deficiency induced functional alterations related to cellular and metabolic processes, and so on. These results demonstrated that proteomics is still an effective tool for the comprehensive exploration of the MSCs differentiation process. cate balance between MSCs differentiation to osteoblasts or adipocytes is finely regulated. To explore novel participating molecules, we screened for early-stage osteogenesis- or adipogenesis-based MSCs protein expression profile using TMT-based quantitative proteomic analysis. Protein annotation, hierarchical clustering, functional stratification, and protein-protein association assessments were performed. Moreover, two upregulated proteins, namely, FBLN2 and NPR3, were validated to participate in the osteogenic differentiation process of MSCs. Subsequently, we independently downregulated FBLN2 and NPR3 during 7 days of osteogenic differentiation, and conducted quantitative proteomics analysis to assess the differential protein regulation between knockdown and control cells. Based on gene ontology (GO) and network analyses, FBLN2 deficiency induced functional alterations associated with biological regulation and stimulus response, whereas, NPR3 deficiency induced functional alterations related to cellular and metabolic processes, and so on. These results demonstrated that proteomics is still an effective tool for the comprehensive exploration of the MSCs differentiation process.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:09:34.103.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianjun Xiong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-07 07:22:08	ID requested
1	2023-10-24 12:27:09	announced
⏵ 2	2023-11-14 09:09:42	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:09:45	announced	2024-10-22: Updated project metadata.

Liu J, He S, Ma B, Li X, Wang Y, Xiong J, TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs). Aging (Albany NY), 15(15):7637-7654(2023) [pubmed]

submitter keyword: marrow mesenchymal stem cells, differentiation, adipogenesis,human, Osteogenesis

Jianjun Xiong
contact affiliation	Basic medical college, Jiujiang University, China
contact email	xiongjj1975@163.com
lab head
Jianjun Xiong
contact affiliation	Jiujiang University
contact email	xiongjj1975@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041404
     1. Label: PRIDE project
     2. Name: TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early MSCs osteogenic differentiation