PXD041402-1

PXD041402 is an original dataset announced via ProteomeXchange.

Title	Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory response
Description	The peri-bronchial zone of chronic obstructive pulmonary disease (COPD) is the site of extensive infiltration of immune cell, allowing persistent contacts between resident cells and immune cells. Tissue fibrocytes interaction with CD8+ T cells and its consequences were investigated. We show that fibrocytes and CD8+ T cells are found in vicinity in distal airways and that potential interactions are more frequent in tissues from COPD patients compared to those of control subjects. Increased proximity and clusterization between CD8+ T cells and fibrocytes are associated with altered lung function. Tissular CD8+ T cells from COPD patients promote fibrocyte chemotaxis via the CXCL8-CXCR1/2 axis. CD8+ T cells establish short-term interactions with fibrocytes, that trigger CD8+ T cell proliferation in a CD54- and CD86-dependent manner, pro-inflammatory cytokines production, CD8+ T cell cytotoxic activity and fibrocyte immunologic signaling. We defined a computational model describing these intercellular interactions and calibrated the parameters based on our experimental measurements. We showed the model’s ability to reproduce histological ex vivo characteristics, and observed major contributions of fibrocyte-mediated CD8+ T cell proliferation and fibrocyte death in COPD development. Using the model to test therapeutic scenarios, we predicted a recovery time of several years, and the failure of targeting independently chemotaxis or interacting processes. Altogether, our study reveals that local interactions between fibrocytes and CD8+ T cells can occur in vivo and could jeopardize the balance between protective immunity and chronic inflammation in bronchi of COPD patients.
HostingRepository	PRIDE
AnnounceDate	2023-07-28
AnnouncementXML	Submission_2023-07-28_08:02:58.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DupuyJean-William
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-07 04:40:47	ID requested
⏵ 1	2023-07-28 08:03:00	announced
2	2023-11-14 09:04:26	announced	2023-11-14: Updated project metadata.

Eyraud E, Maurat E, Sac-Ep, é, e JM, Henrot P, Zysman M, Esteves P, Trian T, Dupuy JW, Leipold A, Saliba AE, Begueret H, Girodet PO, Thumerel M, Hustache-Castaing R, Marthan R, Levet F, Vallois P, Contin-Bordes C, Berger P, Dupin I, T cells in COPD bronchial inflammatory response. Elife, 12():(2023) [pubmed]

submitter keyword: chemotaxis, intercellular interactions, inflammation, remodeling,Chronic respiratory diseases

IsabelleDupin
contact affiliation	Centre de recherche Cardio-Thoracique de Bordeaux, INSERM U1045, PTIB, Hôpital Xavier Arnozan, Avenue du Haut Lévêque, 33604 Pessac Cedex
contact email	isabelle.dupin@u-bordeaux.fr
lab head
DupuyJean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD041402

PRIDE project URI

• PRIDE
  1. PXD041402
     1. Label: PRIDE project
     2. Name: Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory response