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PXD041402 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleShort-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory response
DescriptionThe peri-bronchial zone of chronic obstructive pulmonary disease (COPD) is the site of extensive infiltration of immune cell, allowing persistent contacts between resident cells and immune cells. Tissue fibrocytes interaction with CD8+ T cells and its consequences were investigated. We show that fibrocytes and CD8+ T cells are found in vicinity in distal airways and that potential interactions are more frequent in tissues from COPD patients compared to those of control subjects. Increased proximity and clusterization between CD8+ T cells and fibrocytes are associated with altered lung function. Tissular CD8+ T cells from COPD patients promote fibrocyte chemotaxis via the CXCL8-CXCR1/2 axis. CD8+ T cells establish short-term interactions with fibrocytes, that trigger CD8+ T cell proliferation in a CD54- and CD86-dependent manner, pro-inflammatory cytokines production, CD8+ T cell cytotoxic activity and fibrocyte immunologic signaling. We defined a computational model describing these intercellular interactions and calibrated the parameters based on our experimental measurements. We showed the model’s ability to reproduce histological ex vivo characteristics, and observed major contributions of fibrocyte-mediated CD8+ T cell proliferation and fibrocyte death in COPD development. Using the model to test therapeutic scenarios, we predicted a recovery time of several years, and the failure of targeting independently chemotaxis or interacting processes. Altogether, our study reveals that local interactions between fibrocytes and CD8+ T cells can occur in vivo and could jeopardize the balance between protective immunity and chronic inflammation in bronchi of COPD patients.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDupuy Jean-William
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-07 04:40:47ID requested
12023-07-28 08:03:00announced
22023-11-14 09:04:26announced2023-11-14: Updated project metadata.
Publication List
Eyraud E, Maurat E, Sac-Ep, é, e JM, Henrot P, Zysman M, Esteves P, Trian T, Dupuy JW, Leipold A, Saliba AE, Begueret H, Girodet PO, Thumerel M, Hustache-Castaing R, Marthan R, Levet F, Vallois P, Contin-Bordes C, Berger P, Dupin I, T cells in COPD bronchial inflammatory response. Elife, 12():(2023) [pubmed]
Keyword List
submitter keyword: chemotaxis, intercellular interactions, inflammation, remodeling,Chronic respiratory diseases
Contact List
Isabelle Dupin
contact affiliationCentre de recherche Cardio-Thoracique de Bordeaux, INSERM U1045, PTIB, Hôpital Xavier Arnozan, Avenue du Haut Lévêque, 33604 Pessac Cedex
contact emailisabelle.dupin@u-bordeaux.fr
lab head
Dupuy Jean-William
contact affiliationPlateforme Proteome Bordeaux
contact emailjean-william.dupuy@u-bordeaux.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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