PXD041392-1

PXD041392 is an original dataset announced via ProteomeXchange.

Title	Human gastric micropeptide LC-MS/MS
Description	The Human Proteome Project currently seeks to understand the complexities of functional genomics and proteomics and to systematically identify and functionally characterize noncanonical translated sequences, such as micropeptides, will enrich the understanding of the human genome. By producing a high-coverage micropeptide sequencing reference library with 11,931,202 open reading frames and devising an alternative ultrafiltration-concentrated tandem mass spectrometry assay, we effectively confirmed 10,771 unlabeled proteins. Nearly 40% of them were derived from noncoding RNAs, suggesting that there may be a large number of misannotations in the human genome. These micropeptides exhibited abundance at subcellular locations and broad gastric cancer relevance. CRISPR screening showed that 1161 candidates had critical growth regulatory functions that were associated with their genetic properties. Excitingly, shorter sequenced micropeptides exhibited natural peptide-based drug potential or strong carcinogenic effects. Our findings illustrate a hitherto unrecognized micropeptidome and highlight its valuable implications for cancer therapy and drug development.
HostingRepository	PRIDE
AnnounceDate	2025-01-18
AnnouncementXML	Submission_2025-01-18_04:02:55.521.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041392
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	shaofang xie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-06 22:41:01	ID requested
⏵ 1	2025-01-18 04:02:56	announced

Shi C, Liu F, Su X, Yang Z, Wang Y, Xie S, Xie S, Sun Q, Chen Y, Sang L, Tan M, Zhu L, Lei K, Li J, Yang J, Gao Z, Yu M, Wang X, Wang J, Chen J, Zhuo W, Fang Z, Liu J, Yan Q, Neculai D, Sun Q, Shao J, Lin W, Liu W, Chen J, Wang L, Liu Y, Li X, Zhou T, Lin A, Comprehensive discovery and functional characterization of the noncanonical proteome. Cell Res, ():(2025) [pubmed]

10.6019/PXD041392;

10.1038/s41422-024-01059-3;

submitter keyword: Human, micropeptide

Li Xu
contact affiliation	westlake.edu.cn
contact email	Lixu@westlake.edu.cn
lab head
shaofang xie
contact affiliation	westlake university
contact email	xieshaofang@westlake.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD041392

PRIDE project URI

• PRIDE
  1. PXD041392
     1. Label: PRIDE project
     2. Name: Human gastric micropeptide LC-MS/MS