PXD041386-2

PXD041386 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Simultaneous proteome localization and turnover analysis of cardiomyocytes under proteasome inhibition
Description	To examine the protein spatial and temporal changes upon carfilzomib-mediated proteasome inhibition in cardiac cells, we produced human iPSC-derived cardiomyocytes using a standard small molecule based protocol. Cardiomyocyte identity was confirmed by morphology, observation of contraction, and the presence of GFP tagged MLC-2a in the reporter line. We then applied the SPLAT protocol to untreated and carfilzomib-exposed iPSC-cardiomyocytes.
HostingRepository	jPOST
AnnounceDate	2023-04-28
AnnouncementXML	Submission_2024-09-26_14:26:52.182.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Lau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4); TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-06 10:52:38	ID requested
1	2023-04-27 08:00:06	announced
⏵ 2	2024-09-26 14:26:53	announced	2024-09-27: Updated PubMed.

Publication List

Currie J, Manda V, Robinson SK, Lai C, Agnihotri V, Hidalgo V, Ludwig RW, Zhang K, Pavelka J, Wang ZV, Rhee JW, Lam MPY, Lau E, Simultaneous proteome localization and turnover analysis reveals spatiotemporal features of protein homeostasis disruptions. Nat Commun, 15(1):2207(2024) [pubmed]

Currie J, Manda V, Robinson SK, Lai C, Agnihotri V, Hidalgo V, Ludwig RW, Zhang K, Pavelka J, Wang ZV, Rhee JW, Lam MPY, Lau E, Simultaneous proteome localization and turnover analysis reveals spatiotemporal features of protein homeostasis disruptions. Nat Commun, 15(1):2207(2024) [pubmed]

Keyword List

submitter keyword: iPSC, caridomyocyte, cardiotoxicity, turnover, spatial proteomics

submitter keyword: iPSC, caridomyocyte, cardiotoxicity, turnover, spatial proteomics

Contact List

Edward Lau
lab head
Edward Lau
contact affiliation	University of Colorado
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002119/