PXD041379 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Meta-omics analysis of microbially induced carbonate precipitation at a CO2 rich geyser in Utah |
| Description | Microbially induced carbonate precipitation (MICP) refers to the biogeochemical process in which calcium carbonate is precipitated by altering the local geochemical environment (Mortensen et al. 2011). These alterations occur as a by-product of common microbial metabolic activities by increasing the local carbonate content as well as pH thereby saturating the solution in respect to carbonate. To better understand the microbial ecology of MICP on a community level in natural environments, we chose to evaluate microbial communities derived from travertine adjacent to Crystal Geyser (CG), Utah. CG is a cold-driven, CO2 rich geyser which is surrounded by colorful travertine that has been suggested to be generated through microbial processes. We used a cultivation-independent, multi-omics approach combined with geochemical measurements to investigate metabolic pathways and physiologies potentially involved in MICP at CG. We collected samples from the top 20 cm of travertine adjacent to Crystal Geyser, Utah in November 2019 and June 2021 (38.9384° N, 110.1354° W) wearing gloves at all times. We sampled 1 m away from the borehole (CG-1) and 10 m away from the borehole (CG-10). We preserved all collected samples in RNAlater-like solution (Menke et al., 2017, Front. Microbiol. 8) in a 1:10 sediment: RNAlater-like solution ratio as previously validated (Jensen et al. (2021, Micro. Spec. 2021, 9:2) |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-11-18 |
| AnnouncementXML | Submission_2024-11-18_15:56:24.856.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Marlene Violette |
| SpeciesList | scientific name: Eukaryota (eucaryotes); NCBI TaxID: 2759; scientific name: Archaea; NCBI TaxID: 2157; scientific name: Bacteria; NCBI TaxID: 2; |
| ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-04-06 07:44:04 | ID requested | |
| ⏵ 1 | 2024-11-18 15:56:25 | announced | |
Publication List
Keyword List
| submitter keyword: Rhodobacterales |
| anoxygenic photosynthesis.,MICP |
| Biomineralization |
| Crystal Geyser |
| CaCO3 |
| LC-MS/MS |
| metaproteomics |
| metagenomics |
| travertine formation |
| microbiome |
Contact List
| Manuel Kleiner |
|---|
| contact affiliation | North Carolina State University |
| contact email | manuel_kleiner@ncsu.edu |
| lab head | |
| Marlene Violette |
|---|
| contact affiliation | Duke University |
| contact email | mjensen2@ncsu.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD041379 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041379
- Label: PRIDE project
- Name: Meta-omics analysis of microbially induced carbonate precipitation at a CO2 rich geyser in Utah
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