PXD041365-2

PXD041365 is an original dataset announced via ProteomeXchange.

Title	CKB catalyzes BCAR1 phosphorylation to regulate DNA damage repair
Description	Creatine kinase (CK) is an essential metabolic enzyme mediating creatine/phosphocreatine interconversion and shuttle to replenish ATP for energy needs. Ablation of CK causes deficiency in energy supply that eventually results in reduced muscle burst activity and neurological disorders in mice. Besides the well-established role of CK in energy-buffering, the mechanism underlying non-metabolic function of CK is poorly understood. Here we demonstrate that creatine kinase brain-type (CKB) may function as a protein kinase to regulate BCAR1 Y327 phosphorylation that enhances the association between BCAR1 and RBBP4. Then the complex of BCAR1 and RPPB4 binds to the promoter region of DNA damage repair gene RAD51 and activates its transcription by modulating histone H4K16 acetylation to ultimately promote DNA damage repair. These findings reveal the possible role of CKB independently of its metabolic function and depict the potential pathway of CKB-BCAR1-RBBP4 operating in DNA damage repair.
HostingRepository	iProX
AnnounceDate	2023-04-05
AnnouncementXML	Submission_2023-05-22_00:47:13.264.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bo Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-05 19:55:26	ID requested
1	2023-04-05 19:55:41	announced
⏵ 2	2023-05-22 00:47:14	announced	2023-05-22: Update publication information.

Yang B, Zhang W, Sun L, Lu B, Yin C, Zhang Y, Jiang H, Creatine kinase brain-type regulates BCAR1 phosphorylation to facilitate DNA damage repair. iScience, 26(5):106684(2023) [pubmed]

submitter keyword: creatine kinase brain-type (CKB), BCAR1 phosphorylation, RPPB4, DNA damage repair

Hong Jiang
contact affiliation	Shanghai Institute of Organic Chemistry
contact email	hongjiang@sioc.ac.cn
lab head
Bo Yang
contact affiliation	East China Normal University
contact email	yangbo6674@163.com
dataset submitter

iProX dataset URI