PXD041349-1

PXD041349 is an original dataset announced via ProteomeXchange.

Title	Dll4iDEC mouse liver endothelial cells
Description	Myc and its target Odc1 were among the most 250 strongly upregulated genes in Dll4 mutant ECs, compared with Notch1 and Rbpj mutants. Myc is known to activate important ribosome biogenesis and protein translation pathways, favoring cell growth. Dll4iDEC livers showed upregulation of a large range of canonical E2F, Myc, and mTORC1 target genes and ribosomal genes, particularly in the activated, proliferating, and endothelial tip-cell clusters. This hypermetabolic transcriptional status was confirmed by in-depth proteomics analysis of protein lysates obtained from freshly isolated liver ECs, providing the first proteomics analysis of the endothelial tip-cell state induced by targeting Dll4.
HostingRepository	PRIDE
AnnounceDate	2023-04-11
AnnouncementXML	Submission_2023-04-11_03:14:24.826.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	EmilioCamafeita
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-05 04:57:52	ID requested
⏵ 1	2023-04-11 03:14:25	announced

Dataset with its publication pending

submitter keyword: Dll4iDEC liver endothelial cells, quantitative proteomics

JesúsVázquez
contact affiliation	Cardiovascular Proteomics Laboratory & Proteomics Unit Centro Nacional de Investigaciones Cardiovasculares Madrid, Spain
contact email	jesus.vazquez@cnic.es
lab head
EmilioCamafeita
contact affiliation	Proteomics Unit Vascular Pathophysiology Area CNIC
contact email	ecamafeita@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD041349

PRIDE project URI

• PRIDE
  1. PXD041349
     1. Label: PRIDE project
     2. Name: Dll4iDEC mouse liver endothelial cells