PXD041348 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach |
Description | S-Sulfocysteine (SSC), a bioavailable L-cysteine derivative (Cys), is known to be taken up and metabolized in Chinese hamster ovary (CHO) cells used to produce novel therapeutic biological entities. To gain a deeper mechanistic insight into the SSC biological activity and metabolization, a comprehensive multi-omics study was performed on industrially relevant CHO-K1 GS cells throughout a fed-batch process, including metabolomic and proteomic profiling combined with multivariate data and pathway analyses. Multi-layered data and enzymatical assays revealed an intracellular SSC/glutathione mixed disulfide formation and glutaredoxin-mediated reduction, releasing Cys and sulfur species. Increased Cys availability was directed towards glutathione and taurine synthesis, while other Cys catabolic pathways were likewise affected, indicating the cells strive to maintain Cys homeostasis and cellular functions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-08-03 |
AnnouncementXML | Submission_2023-08-03_12:03:20.562.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD041348 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | MelanieNguyen |
SpeciesList | scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-05 04:49:47 | ID requested | |
⏵ 1 | 2023-08-03 12:03:21 | announced | |
2 | 2023-11-14 07:00:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: redox control, omics, CHO,S-sulfocysteine, biological activity |
Contact List
Dr. AlineZimmer |
contact affiliation | Upstream R&D, Merck Life Science KGaA, Frankfurter Straße 250, 64293 Darmstadt, Germany |
contact email | aline.zimmer@merckgroup.com |
lab head | |
MelanieNguyen |
contact affiliation | Merck Life Science KGaA |
contact email | melanie.nguyen@external.merckgroup.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041348
- Label: PRIDE project
- Name: Mechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach