PXD041318-1

PXD041318 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteomic profiling of aqueous humor in idiopathic uveitis and Vogt-Koyanagi-Harada syndrome
Description	In this study, 44 AH samples were subjected to a quantitative analysis using SWATH, and 557 proteins were identified in each sample according to a well-established library of 634 proteins. Proteomic profiles in AH of IU and VKH groups were significantly different from normal controls, whereas relatively minor inter-group variation was observed. Differential analysis revealed a shared pattern of extracellular matrix disruption in IU and VKH groups and, more importantly, downregulation of retinal cellular proteins highlighting the value of AH as a medium for ocular disease research. Enrichment analysis showed a typical inflammatory state in the protein composition of AH in IU and VKH groups compared to normal controls, where innate immunity played an important role, as indicated by the activation of the complement cascade and the overexpression of innate immune cell markers. Subsequently, an efficient and robust machine learning algorithm, XGBoost, was used to develop a model for classifying IU, VKH, and normal controls. The top three ranked proteins in the importance list, TF, ENPP2, and FUCA1, were found to represent a promising biomarker panel.
HostingRepository	iProX
AnnounceDate	2023-04-04
AnnouncementXML	Submission_2023-04-03_21:28:15.296.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lingzi Wu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-03 21:28:07	ID requested
⏵ 1	2023-04-03 21:28:15	announced
2	2024-06-06 00:52:03	announced	2024-06-06: Update information.

Dataset with its publication pending

submitter keyword: Idiopathic uveitis, VKH, proteomic, aqueous humor, XGBoost

Xiaomin Zhang
contact affiliation	Tianjin Medical University Eye Hospital
contact email	xzhang08@tmu.edu.cn
lab head
Lingzi Wu
contact affiliation	Tianjin Medical University Eye Hospital
contact email	wlz_5206@tmu.edu.cn
dataset submitter

iProX dataset URI