PXD041297-1
PXD041297 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | C1improves motor performance and muscle pathology via activating AMPK-PGC-1α in a mouse model of Duchenne muscular dystrophy |
Description | Objectives: This study was conducted to investigate the protective effect and intervention mechanism of C1 (a selective AMPK activator) on muscle damage in mice with Duchenne muscular dystrophy (DMD). Methods: We administered C1 (18 mg/kg and 54 mg/kg) to 10-week-old male DMD mice for 8 weeks. A series of behavioral tests were carried out to assess motor ability. Skeletal muscle samples were subjected to pathological analysis, western blot and proteomic analysis to explore the different protein networks associated with muscle pathology. Compound C, an AMPKα inhibitor, has been used as a negative control for follow-up experiments to verify the efficiency of C1 on AMPK activation. Key findings: C1 treatment significantly enhanced motor function and reduced muscle fibrosis and inflammation in DMD mice. We then found that C1 activated the AMPKα/PGC-1α signaling pathway, increasing ATP levels and mitochondrial DNA copy number in DMD mice. These improved effects on mitochondrial function were reversed by Compound C. In addition, Gastrocnemius muscle proteomics showed that C1 attenuated oxidative stress damage and improved muscle structural development. Conclusion: Collectively, C1 alleviates muscle damage in DMD mice by activating AMPKα to improve mitochondria. |
HostingRepository | iProX |
AnnounceDate | 2023-04-03 |
AnnouncementXML | Submission_2023-04-03_00:33:21.383.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xiao Yang |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | trimethylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-04-03 00:33:07 | ID requested | |
⏵ 1 | 2023-04-03 00:33:21 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Duchenne muscular dystrophy, Fibrosis, Inflammation, Oxidative damage, Mitochondria, AMPK |
Contact List
Xifei Yang | |
---|---|
contact affiliation | Shenzhen Center for Disease Control and Prevention |
contact email | xifeiyang@gmail.com |
lab head | |
Xiao Yang | |
contact affiliation | University of South China |
contact email | 2584620446@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |