PXD041296-1

PXD041296 is an original dataset announced via ProteomeXchange.

Title	Functional viromic screens uncover regulatory RNA elements
Description	The number of sequenced viral genomes has surged recently, presenting an opportunity to understand viral diversity and uncover unknown regulatory mechanisms. Here we conducted a screening of 30,367 viral segments from 143 species representing 96 genera and 37 families. Using a library of viral segments in 3′ UTR, we identified hundreds of elements impacting RNA abundance, translation, and nucleocytoplasmic distribution. To illustrate the power of this approach, we investigated K5, an element conserved in kobuviruses, and found its potent ability to enhance mRNA stability and translation in various contexts, including adeno-associated viral vectors and synthetic mRNAs. Moreover, we identified a previously uncharacterized protein, ZCCHC2, as a critical host factor for K5. ZCCHC2 recruits the terminal nucleotidyl transferase TENT4 to elongate poly(A) tails with mixed sequences, delaying deadenylation. This study provides a unique resource for virus and RNA research and highlights the potential of the virosphere for biological discoveries.
HostingRepository	PRIDE
AnnounceDate	2023-06-23
AnnouncementXML	Submission_2023-06-23_01:53:09.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JennySeo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-03 00:18:34	ID requested
⏵ 1	2023-06-23 01:53:09	announced
2	2023-11-14 06:47:26	announced	2023-11-14: Updated project metadata.

submitter keyword: Human, RAPID, LC-MS/MS

V. NarryKim
contact affiliation	School of Biological Sciences, Seoul National University Center for RNA Research, Institute for Basic Science
contact email	narrykim@snu.ac.kr
lab head
JennySeo
contact affiliation	School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea.
contact email	rjenny204@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041296

PRIDE project URI

• PRIDE
  1. PXD041296
     1. Label: PRIDE project
     2. Name: Functional viromic screens uncover regulatory RNA elements