PXD041291-2
PXD041291 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap |
Description | In this work, a LPIT-reversed phase liquid chromatography-tandem mass spectrometry (LPIT-RPLC-MS/MS) platform was established for deep bottom-up proteomics. LPIT was used here as a robust and effective method for peptide fractionation. LPIT separates peptides based on their effective charges and hydrodynamic radii, which is orthogonal to that of RPLC. With excellent orthogonality, the integration of LPIT with RPLC-MS/MS could effectively increase the number of peptide and protein being detected. When analyzing HeLa cells, peptide and protein coverages were increased by ~89.2% and 50.3%, respectively. With high efficiency and low cost, this LPIT based peptide fraction method could potentially be used in routine deep bottom-up proteomics. |
HostingRepository | iProX |
AnnounceDate | 2023-04-03 |
AnnouncementXML | Submission_2024-06-06_00:52:44.813.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xinyan Fu |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-04-02 21:58:31 | ID requested | |
1 | 2023-04-02 21:58:45 | announced | |
⏵ 2 | 2024-06-06 00:52:45 | announced | 2024-06-06: Update information. |
Publication List
Fu X, Hong J, Zhai Y, Liu K, Xu W, Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap. Anal Chem, 95(27):10137-10144(2023) [pubmed] |
Keyword List
submitter keyword: bottom-up proteomics, liquid phase ion trap, mass spectrometry, peptide fractionation |
Contact List
Wei Xu | |
---|---|
contact affiliation | Beijing Institute of Technology |
contact email | weixu@bit.edu.cn |
lab head | |
Xinyan Fu | |
contact affiliation | Beijing Institute of Technology |
contact email | 1121428828@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |