PXD041237-2

PXD041237 is an original dataset announced via ProteomeXchange.

Title	Heat-killed probiotic Levilactobacillus brevis MRKAK9 downregulates insulin-signaling pathway and improves proteostasis and autophagy to promote longevity and immunity in Caenorhabditis elegans
Description	Probiotics play important role in maintaining the health and extend longevity in their host. Previous studies reported several live probiotic bacteria in enhancing longevity and improving diverse feature of the host’s health. In this study, we reported a new potential heat- killed probiotic bacterium Levilactobacillus brevis strain MRKAK9 improved longevity and different features of healthy aging, including age-associated physical activity, improved resistance to biotic and abiotic stress in C. elegans. The mechanistic investigations showed that heat-killed strain MRKAK9 promoted longevity and healthy aging by downregulating insulin-signaling pathway resulting in improved proteostasis, autophagy and preserving lysosomal functionality in C. elegans. Heat-killed strain MRKAK9-mediated downregulation of insulin signaling pathway was regulated by the miRNA mir-243, suggesting that mir-243 partially involved in enhancing longevity of C. elegans. Additionally, the structural component exopolysaccharide of the strain heat-killed MRKAK9 also downregulated insulin- signaling mechanisms, increased the expression of genes involved in proteostatsis, autophagy as well as improved expression of mir-243. This study indicates that heat-killed strain MRKAK9 improves longevity and healthy aging, suggesting its candidature as a novel postbiotic.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:54:08.359.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mojibur R. Khan
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-31 01:06:32	ID requested
1	2024-08-10 12:04:40	announced
⏵ 2	2024-10-22 06:54:15	announced	2024-10-22: Updated project metadata.

Kumar A, Saha MK, Kumar V, Bhattacharya A, Barge S, Mukherjee AK, Kalita MC, Khan MR, Heat-killed probiotic Levilactobacillus brevis MKAK9 and its exopolysaccharide promote longevity by modulating aging hallmarks and enhancing immune responses in Caenorhabditis elegans. Immun Ageing, 21(1):52(2024) [pubmed]

10.1186/s12979-024-00457-w;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Food and Nutrition (B/D-HPP), Human Proteome Project

submitter keyword: longevity

lactic acid bacteria

aging biomarkers

SKN-1 transcription factor

p38 MAPK signaling

behavior

intestinal integrity

Dr. Mojibur R. Khan
contact affiliation	Associate Professor, Institute of Advanced Study in Science and Technology (IASST), Guwahati-110035, Assam, India
contact email	mblab.iasst@gmail.com
lab head
Mojibur R. Khan
contact affiliation	Institute of Advanced Studt in Science and Technology (IASST)
contact email	mblab.iasst@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD041237

PRIDE project URI

• PRIDE
  1. PXD041237
     1. Label: PRIDE project
     2. Name: Heat-killed probiotic Levilactobacillus brevis MRKAK9 downregulates insulin-signaling pathway and improves proteostasis and autophagy to promote longevity and immunity in Caenorhabditis elegans