PXD041229-1

PXD041229 is an original dataset announced via ProteomeXchange.

Title	The genetic and dietary landscape of the muscle insulin signalling network
Description	Using mass spectrometry-based phosphoproteomics, we quantified 23,126 phosphosites in the skeletal muscle of five genetically distinct inbred mouse strains exposed to two controlled dietary environments, with and without acute insulin treatment. Almost half of the insulin-regulated phosphoproteome was altered by genetic background independently of diet, and high-fat high-sugar feeding also affected insulin signalling in a strain-dependent manner. Our data illuminated signalling network organisation principles, including the uncoupling of phosphosites targeted by the same kinase. Associating diverse signalling responses with insulin-stimulated glucose uptake uncovered regulators of muscle insulin responsiveness, including the regulatory phosphosite S469 on Pfkfb2, a key glycolytic enzyme.
HostingRepository	PRIDE
AnnounceDate	2023-12-21
AnnouncementXML	Submission_2023-12-21_04:29:18.158.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean Humphrey
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-30 21:20:58	ID requested
⏵ 1	2023-12-21 04:29:18	announced

Dataset with its publication pending

submitter keyword: phosphoproteomics, glucose regulation, phosphoproteome, diet, environment, genetic, skeletal muscle, mouse

Sean Humphrey
contact affiliation	Functional Phosphoproteomics, Murdoch Children's Research Institute, Australia
contact email	sean.humphrey@mcri.edu.au
lab head
Sean Humphrey
contact affiliation	Murdoch Children's Research Institute
contact email	sean.j.humphrey@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD041229

PRIDE project URI

• PRIDE
  1. PXD041229
     1. Label: PRIDE project
     2. Name: The genetic and dietary landscape of the muscle insulin signalling network