PXD041209 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The impact of culture conditions on E. coli acetylome and proteome |
Description | A proteome and acetylome study under different culture conditions was carried out by quantitative label-free mass spectrometry analysis. E. coli K12 BW25113 strain was grown employing TB7 complex medium or M9 minimal medium, supplemented with glucose 20 mM or glycerol 40 mM as carbon source. Therefore, 4 culture conditions (TB7-glucose, TB7-glycerol, MM9-glucose, and MM9-glycerol) were used. Samples were taken in exponential and in stationary growth phase, resulting in 8 experimental samples: TB7-glucose in exponential phase, TB7-glucose in stationary phase, TB7-glycerol in exponential phase, TB7-glycerol in stationary phase, MM9-glucose in exponential phase, MM9-glucose in stationary phase, MM9-glycerol in exponential phase, and MM9-glycerol in stationary phase, with 4 biological replicates from each. This work emphasises the importance of the culture conditions choice, as they determine both, location of acetylation, and acetylation level, which may have an impact on regulation of the central metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-21 |
AnnouncementXML | Submission_2024-05-21_07:30:48.248.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Teresa de Diego Puente |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
ModificationList | acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-30 04:22:06 | ID requested | |
⏵ 1 | 2024-05-21 07:30:49 | announced | |
Publication List
10.1016/j.isci.2024.109017; |
Lozano-Terol G, Chiozzi RZ, Gallego-Jara J, Sola-Mart, í, nez RA, Vivancos AM, Ortega Á, Heck AJR, D, í, az MC, de Diego Puente T, protein acetylome. iScience, 27(2):109017(2024) [pubmed] |
Keyword List
ProteomeXchange project tag: EPIC-XS |
submitter keyword: E. coli, LC-MS, PTM,Lysine acetylation, fluxes, metabolism regulation |
Contact List
Teresa de Diego Puente |
contact affiliation | Departamento de Bioquímica y Biología Molecular B e Inmunologia. Facultad de Química. Universidad de Murcia. Campus de Excelencia Mare Nostrum |
contact email | tdp@um.es |
lab head | |
Teresa de Diego Puente |
contact affiliation | Department of Biochemistry and Molecular Biology B and Immunology. Faculty of Chemistry. University of Murcia. EUniWell. Mare Nostrum Campus of Excellence. |
contact email | tdp@um.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041209
- Label: PRIDE project
- Name: The impact of culture conditions on E. coli acetylome and proteome