PXD041196-1

PXD041196 is an original dataset announced via ProteomeXchange.

Title	Ubiquitination of the actin polymerase VASP
Description	Filopodia are dynamic, actin-rich structures that extend outward from the cell to explore and respond to cues in the local environment. The actin polymerase VASP is a component of the filopodial tip complex, where it regulates actin polymerization and filopodial dynamics. Previously, we showed that VASP transiently co-localizes with the brain-enriched E3 ubiquitin ligase TRIM9 at the tips of neuronal filopodia. TRIM9 was required for the reversible, non-degradative ubiquitination of VASP and this modification was associated with decreased filopodia number and stability. Furthermore, the axon guidance cue netrin promoted deubiquitination of VASP. We hypothesize mono or multi-monoubiquitination of VASP is a mechanism to negatively regulate actin dynamics by blocking VASP and actin interactions. To determine the lysine residues that are ubiquitinated in VASP, we immunoprecipitated exogenously expressed human VASP from HEK293 cells. The immunopurified protein was analyzed by mass spectrometry to identify post-translational modifications. We identified numerous lysine residues that are ubiquitinated in VASP, including a high prevalence at K240 and K286.
HostingRepository	PRIDE
AnnounceDate	2024-01-17
AnnouncementXML	Submission_2024-01-17_06:42:54.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aurora Cabrera
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine; phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-30 01:17:00	ID requested
⏵ 1	2024-01-17 06:42:54	announced
2	2024-10-22 06:22:54	announced	2024-10-22: Updated project metadata.

10.1242/JCS.261527;

submitter keyword: Actin

Ubiquitin

VASP

Post-translational modification

Stephanie Gupton
contact affiliation	UNC Chapel Hill
contact email	sgupton@email.unc.edu
lab head
Aurora Cabrera
contact affiliation	Department of Pharmacology at UNC Chapel Hill
contact email	Aurora_Cabrera@med.unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041196
     1. Label: PRIDE project
     2. Name: Ubiquitination of the actin polymerase VASP