PXD041168 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Acute deletion of the glucocorticoid receptor in hepatocytes disrupts postprandial lipid metabolism |
Description | Hepatic lipid metabolism is highly dynamic, and disruption of several circadian transcriptional regulators results in hepatic steatosis. This includes genetic disruption of the glucocorticoid receptor (GR) as the liver develops. To address the functional role of GR in the adult liver, we used an acute hepatocyte-specific GR knockout model (hepGRKO) to study temporal hepatic lipid metabolism governed by GR at several pre- and postprandial timepoints. Lipidomics analysis revealed significant temporal lipid metabolism, with GR disruption resulting in impaired regulation of specific triglycerides, non-esterified fatty acids, and sphingolipids. This correlated with increased number and size of lipid droplets and mildly reduced mitochondrial respiration, most noticeably in the postprandial phase. Proteomics and transcriptomics analyses suggest that dysregulated lipid metabolism originates from pronounced induced expression of enzymes involved in fatty acid synthesis, -oxidation, and sphingolipid metabolism. Integration of GR cistromic data suggests that induced genes are a result of regulatory actions secondary to direct GR effects on transcription. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:05:02.505.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Felix Boel Pedersen |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-28 08:20:04 | ID requested | |
1 | 2023-09-07 09:56:28 | announced | |
⏵ 2 | 2023-11-14 09:05:05 | announced | 2023-11-14: Updated project metadata. |
Publication List
Correia CM, Pr, æ, stholm SM, Havelund JF, Pedersen FB, Siersb, æ, k MS, Ebbesen MF, Gerhart-Hines Z, Heeren J, Brewer J, Larsen S, Blagoev B, F, æ, rgeman NJ, Gr, ø, ntved L, Acute Deletion of the Glucocorticoid Receptor in Hepatocytes Disrupts Postprandial Lipid Metabolism in Male Mice. Endocrinology, 164(10):(2023) [pubmed] |
Keyword List
submitter keyword: Glucocorticoid Receptor knockout mice, DIA,Mouse, Lipid Metabolism, Circadian rhythm, Liver, LC-MS/MS |
Contact List
Blagoy Blagoev |
contact affiliation | Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark |
contact email | bab@bmb.sdu.dk |
lab head | |
Felix Boel Pedersen |
contact affiliation | University of Southern Denmark |
contact email | felixbp@bmb.sdu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041168
- Label: PRIDE project
- Name: Acute deletion of the glucocorticoid receptor in hepatocytes disrupts postprandial lipid metabolism