PXD041082-1

PXD041082 is an original dataset announced via ProteomeXchange.

Title	Palaeoproteomics of archaeological shell beads
Description	In this project we have applied palaeoproteomics to study archaeological shell beads dating back to the beginning of the 7th mill. cal BCE and recovered from a prehistoric site in Jordan (Ba'ja). These beads were made of mollusc shells and were of unknown origin, but suspected to be made of larger bivalves such as Tridacna or Spondylus sp. The aim of the study was to analyse ancient proteins extracted from these beads in order to infer their biological origin. Tiny amounts of ancient samples were used and the extracted proteins were analysed by high resolution liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). In order to identify obtained peptides, we have created an ‘in-house’ molluscan protein database, by gathering molluscan sequences (proteins and transcriptomes), which were downloaded from publicly available databases in NCBI. Searching against this comprehensive database, we were able to identify a number of shell proteins from Tridacna sp., the giant clam shells. Our study reports the oldest molluscan protein sequences ever recovered that come from a very warm environment.
HostingRepository	PRIDE
AnnounceDate	2024-11-04
AnnouncementXML	Submission_2024-11-04_03:28:01.164.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041082
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jorune Sakalauskaite
SpeciesList	scientific name: Mollusca; NCBI TaxID: 6447;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-24 01:27:55	ID requested
⏵ 1	2024-11-04 03:28:02	announced

10.6019/PXD041082;

Alarashi H, Benz M, Gresky J, Burkhardt A, Fischer A, Gourichon L, Gerlitzki M, Manfred M, Sakalauskaite J, Demarchi B, Mackie M, Collins M, Odriozola CP, Garrido Cordero J Á, Avil, é, s M Á, Vigorelli L, Re A, Gebel HGK, Threads of memory: Reviving the ornament of a dead child at the Neolithic village of Ba`ja (Jordan). PLoS One, 18(8):e0288075(2023) [pubmed]

10.1371/journal.pone.0288075;

submitter keyword: archaeological shells, mollusc shells, ancient proteins,Palaeoproteomics, LC-MS/MS

Matthew J. Collins
contact affiliation	Section of Geobiology, The Globe Institute, Faculty of Health and Medical Sciences, University of Copenhagen
contact email	matthew@palaeome.org
lab head
Jorune Sakalauskaite
contact affiliation	Life Sciences Centre, Vilnius University
contact email	jorune@palaeome.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD041082

PRIDE project URI

• PRIDE
  1. PXD041082
     1. Label: PRIDE project
     2. Name: Palaeoproteomics of archaeological shell beads