PXD041077-2

PXD041077 is an original dataset announced via ProteomeXchange.

Title	Human fibroblast-like synoviocytes (FLSs) -derived secretome
Description	Invasive pannus, mainly composed of fibroblast-like synoviocytes (FLSs), is a hallmark of rheumatoid arthritis (RA) pathology. Secreted proteins from RA-FLS play key roles in RA invasive pannus. However, RA-FLS-derived secretome associated with invasive pannus has not been systematically investigated. Here, we first identified 843 secreted proteins from RA-FLSs treated with TNFα and IL-1β using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Functional enrichment analysis revealed that 58.5% (493 proteins) of the secretome were associated with pannus-driven RA pathologies. Among them, parallel reaction monitoring (PRM) analysis then identified 16 secreted proteins that were increased in RA SFs (117 samples) than in OA SFs (45 samples). Of them, MYH9 further showed significant positive correlations with RA pathological parameters, such as synovial hyperplasia, increased articular vascularity, and inflammation severity. Molecular and cellular experiments confirmed that MYH9 was expressed in RA-FLSs and more highly under disease-aggravating conditions, and MYH9 depletion significantly defected migration and invasion of RA-FLS in RA pannus. Our study provides a comprehensive resource of RA-FLS-derived secretome, and our results suggest MYH9 that was increased in RA SF and strongly correlated with disease severity as a potential therapeutic target for invasive pannus.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:47.456.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sehyun Chae
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-23 23:15:07	ID requested
1	2023-06-29 22:31:39	announced
⏵ 2	2023-11-14 09:01:50	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:51:30	announced	2024-10-22: Updated project metadata.

Lee S, Choi E, Chae S, Koh JH, Choi Y, Kim JG, Yoo SA, Hwang D, Kim WU, Identification of MYH9 as a key regulator for synoviocyte migration and invasion through secretome profiling. Ann Rheum Dis, 82(8):1035-1048(2023) [pubmed]

submitter keyword: Rheumatoid arthritis, Secretome, Fibroblast-like synoviocytes

Sehyun Chae
contact affiliation	Neurovascular Unit Research Group, Korean Brain Research Institute, Republic of Korea
contact email	shchae@kbri.re.kr
lab head
Sehyun Chae
contact affiliation	Korea Brain Research institute
contact email	shchae@kbri.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041077
     1. Label: PRIDE project
     2. Name: Human fibroblast-like synoviocytes (FLSs) -derived secretome