PXD041046-1

PXD041046 is an original dataset announced via ProteomeXchange.

Title	SAPrIm, A Semi-Automated Protocol for mid-throughput Immunopeptidomics
Description	Human leukocyte antigen (HLA) molecules play a crucial role in the development of adaptive immune responses and therefore have been of major interest in the development of immunotherapies such as cancer vaccines and chimeric antigen receptor (CAR) T cells. Hence, a comprehensive understanding and profiling of the immunopeptidome is required to foster growth for these personalised solutions. We herein describe a novel immunopeptidomics workflow involving the Kingfisher platform (Thermo Scientific) to isolate immunopeptidomes with anti-HLA antibodies coupled to a proprietary hyper-porous protein A microparticle (MagReSyn®), and a modified variable window data independent acquisition (DIA) method. Using the workflow, we were able to identify ~1500 peptides in as low as 5e6 cells. Overall, the robustness of this workflow will be very important for the future of the immunopeptidome profiling, especially for smaller sample types, and will be a useful tool for the development of immunotherapies and other precision medicine approaches.
HostingRepository	PRIDE
AnnounceDate	2023-05-23
AnnouncementXML	Submission_2023-05-22_17:27:57.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TerryLim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-23 04:47:36	ID requested
⏵ 1	2023-05-22 17:27:57	announced
2	2024-10-22 05:45:50	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Immunopeptidomics, Human leukocyte antigen, Kingfisher

PouyaFaridi
contact affiliation	1. Monash Proteomics and Metabolomics Facility, Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia 2. School of Clinical Sciences, Department of Medicine, Monash University, Clayton, Victoria, Australia
contact email	pouya.faridi@monash.edu
lab head
TerryLim
contact affiliation	Monash University
contact email	terry.lim@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD041046

PRIDE project URI

• PRIDE
  1. PXD041046
     1. Label: PRIDE project
     2. Name: SAPrIm, A Semi-Automated Protocol for mid-throughput Immunopeptidomics