PXD041041-1
PXD041041 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Serum O-glycoproteome using O-glycopeptide truncation strategy |
Description | Here, we report an O-glycopeptide truncation strategy for the characterization of protein O-GalNAcylation in biological samples. The O-glycopeptide truncation strategy utilizes another protease or O-glycopeptidase for targeted cleavage of tryptic and enriched O-glycopeptides, which increases the analytical coverage of O-GalNAc glycopeptides and glycoproteins. Tryptic O-glycopeptides covered with dense O-glycan clusters and terminal sialic acids could be well isolated by the hydrophilic-based enrichment approaches. And the enriched O-glycopeptides are then enzymatically truncated into shorter or less multiply O-glycosylated peptides, which are more favorable for MS detection and database search in general bottom-up glycoproteomics. We exploit different enzymatic schemes in the O-glycopeptide truncation strategy for large-scale O-glycoproteome analysis. Ultimately, we comprehensively identify nearly 2000 O-glycopeptides from 391 glycoproteins in a total of 75 µL human serum. Moreover, the truncated O-glycopeptides contribute to site-specific O-glycosylation characterization in stepped high-energy collisional dissociation fragmented LC-MS/MS. Together, the O-glycopeptide truncation strategy has great potential to facilitate the in-depth study of O-GalNAc glycoproteomics in biological samples. |
HostingRepository | jPOST |
AnnounceDate | 2023-05-13 |
AnnouncementXML | Submission_2023-05-12_22:49:28.882.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luyao |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; deamidated L-asparagine; deamidated L-glutamine |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-03-23 02:57:45 | ID requested | |
⏵ 1 | 2023-05-12 22:49:29 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: human serum, O-GalNAc glycosylation |
Contact List
Luyao Liu, Mingliang Ye | |
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lab head | |
Luyao | |
contact affiliation | CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS) |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
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