PXD041029 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide |
| Description | Desmocollin-1 (DSC1) is a desmosomal transmembrane glycoprotein that maintains cell-to-cell adhesion. DSC1 was previously associated with lymph node metastasis of luminal A breast tumors and was found to increase metastatic potential of MCF7 cells in vitro. To delineate DSC1 role in breast cancer metastasis and evaluate possibilities of DSC1 modulation, we investigated the effect of DSC1 overexpression on morphology, cell survival, transcriptome, proteome and interactome of MCF7 cells, a luminal A breast cancer model, stably transduced with lentiviral vector carrying DSC1 gene (MCF7-DSC1-GFP). We moreover identified inhibitor parthenolide to decrease DSC1 protein levels and to modulate the molecular mechanisms associated with DSC1 in MCF7 cells. This PRIDE project includes quantitative analysis results for the total proteome LC-DIA-MS/MS experiment evaluating DSC1 overexpression and parthenolide treatment in MCF7 breast cancer cell line, and results of pulldown analysis of DSC1-interacting proteins in MCF7 cells with and without parthenolide treatment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_06:09:59.197.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pavel Bouchal |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-03-22 06:11:24 | ID requested | |
| 1 | 2023-10-24 12:44:35 | announced | |
| 2 | 2023-11-14 09:10:40 | announced | 2023-11-14: Updated project metadata. |
| ⏵ 3 | 2024-10-22 06:10:00 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.1186/s11658-023-00481-6; |
| Lapcik P, Sulc P, Janacova L, Jilkova K, Potesil D, Bouchalova P, M, ΓΌ, ller P, Bouchal P, Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide. Cell Mol Biol Lett, 28(1):68(2023) [pubmed] |
Keyword List
| submitter keyword: pulldown, breast cancer, proteomics,DIA, DSC1, metastasis |
Contact List
| Pavel Bouchal |
|---|
| contact affiliation | Masaryk University, Faculty of Science |
| contact email | bouchal@chemi.muni.cz |
| lab head | |
| Pavel Bouchal |
|---|
| contact affiliation | Masaryk University, Faculty of Science |
| contact email | bouchal@chemi.muni.cz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD041029 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041029
- Label: PRIDE project
- Name: Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide
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