PXD040987-1
PXD040987 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | AI-facilitated multi-omics reveal TRF-controlled propionylation in determining protein homeostasis |
| Description | Firstly, we systematically identified rhythmically modified Kpr sites, through time-series propionylomic profiling of fly heads. Wild-type (WT) and per0 flies were entrained under light/dark cycles (LD) and then released into constant darkness (DD), respectively. On the first day of DD, whole heads of WT flies were collected every 6 hours, including Zeitgeber time 0 (ZT0), ZT6, ZT12 and ZT18, whereas per0 fly heads were collected at ZT6 and ZT18, respectively. For each sample, whole-cell lysates were digested by trypsin and labeled with one of the tandem mass tag (TMT) 6-plex reagents. Then, propionylated peptides were isolated by immuno-affinity enrichment using the pan-propionylation antibody. The 6 samples were mixed with equal amounts and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Secondly, metabolomic profiling was conducted using TRF-WT and TRF-H2BK17A fly heads collected at ZT9 and ZT21. After the preprocessing including homogenization and different reagents mixture followed by centrifugation. All samples were analyzed by the LC-MS/MS system. Additionally, Immunoprecipitaion and LC-MS/MS analysis was perform with fly heads using H2BK17pr antibody to evaluate the its enrichment capacity. |
| HostingRepository | iProX |
| AnnounceDate | 2023-03-20 |
| AnnouncementXML | Submission_2023-03-20_19:20:51.442.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chenwei Wang |
| SpeciesList | scientific name: Drosophila melanogaster; NCBI TaxID: 7227; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus; X500R QTOF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-03-20 19:20:13 | ID requested | |
| ⏵ 1 | 2023-03-20 19:20:51 | announced | |
| 2 | 2023-05-22 00:45:09 | announced | 2023-05-22: Update publication information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Drosophila, propionylation, metabolome, per |
Contact List
| Yu Xue | |
|---|---|
| contact affiliation | Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology and the Collaborative Innovation Center for Brain Science, Huazhong University of Science and Technology |
| contact email | xueyu@hust.edu.cn |
| lab head | |
| Chenwei Wang | |
| contact affiliation | Huazhong University of Science and Technology |
| contact email | reviewerfly2018@gmail.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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