PXD040959-1

PXD040959 is an original dataset announced via ProteomeXchange.

Title	Profiling of serum N-glycome in ovarian cancer by MALDI-MS
Description	Ovarian cancer (OC) is the one of the most common gynecological tumors with high morbidity and mortality. Alterations in serum N-glycosylation have been observed in many diseases, and specific N-glycans of serum could be used for disease diagnosis or prognosis. However, the association between serum N-glycome profiles and OC progression remains unclear. Herein, high-throughput mass spectrometry was applied to characterize serum N-glycome profile in individuals with healthy controls, benign neoplasm and different stages of OC patients. It was observed that high-mannosylation, neutral bisection and agalactosylation of serum glycoproteins were uniformly increased in the initiation and development of OC, but sialylation was substantially changed on an opposite trend. Additionally, agalactosylation, high-mannosylation and sialylation gained at least moderately accurate merit for diagnosis of both OC and benign neoplasm. Notably, serum N-glycome profile could accurately discriminate OC patients from benign cohorts, with a comparable or even higher diagnostic score compared with CA125 and HE4. Furthermore, bioinformatics verified the differential expression of glycogens in OC progression. Significantly, strong correlations were found between CA 125 and high-mannosylation, galactosylation and sialylation. These findings demonstrated the great potential of serum N-glycome for OC diagnosis and precancerous lesion prediction, paving a new way for OC screening and monitoring.
HostingRepository	PRIDE
AnnounceDate	2023-11-24
AnnouncementXML	Submission_2023-11-24_05:28:57.641.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Si Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-18 20:13:32	ID requested
⏵ 1	2023-11-24 05:28:58	announced

Dataset with its publication pending

submitter keyword: ovarian cancer, N-glycans,MALDI-MS,diagnosis,serum

Xin Liu
contact affiliation	Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, N0. 1037 Luoyudong Road, Hongshan District, Wuhan 430074, China.
contact email	xliu@mail.hust.edu.cn
lab head
Si Liu
contact affiliation	Huazhong University of Science and Technology
contact email	siliu2019@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD040959

PRIDE project URI

• PRIDE
  1. PXD040959
     1. Label: PRIDE project
     2. Name: Profiling of serum N-glycome in ovarian cancer by MALDI-MS