PXD040957-1

PXD040957 is an original dataset announced via ProteomeXchange.

Title	Proteomics of immune cells from liver tumors reveals immunotherapy targets
Description	Elucidating the mechanisms by which immune cells become dysfunctional in tumors is critical to developing next-generation immunotherapies. We profiled proteomes of cancer cells, monocyte/macrophages, CD4+ and CD8+ T cells, and NK cells isolated from tumors, liver, and blood of 48 patients with hepatocellular carcinoma. We found that tumor macrophages induce the sphingosine-1-phospate-degrading enzyme SGPL1, which dampened their inflammatory phenotype and anti-tumor function in vivo. We further discovered that the signaling scaffold protein AFAP1L2, typically only found in activated NK cells, is also upregulated in chronically stimulated CD8+ T cells in tumors. Ablation of AFAP1L2 in CD8+ T cells increased their viability upon repeated stimulation and enhanced their antitumor activity synergistically with PD-L1 blockade in mouse models. Our data revealed new targets for immunotherapy and provide a resource on immune cell proteomes in liver cancer (www.immunomics.ch/liver).
HostingRepository	PRIDE
AnnounceDate	2023-04-20
AnnouncementXML	Submission_2023-04-19_16:20:32.668.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MatteoPecoraro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-17 16:09:03	ID requested
⏵ 1	2023-04-19 16:20:33	announced

Dataset with its publication pending

submitter keyword: NK cells, macrophages, immunotherapy, CD8,Hepatocellular carcinoma, T cells, CD4, monocytes

RogerGeiger
contact affiliation	Institute for Research in Biomedicine (IRB), Università della Svizzera italiana, Bellinzona, Switzerland
contact email	roger.geiger@irb.usi.ch
lab head
MatteoPecoraro
contact affiliation	Institute for Research in Biomedicine, Via Vincenzo Vela 6 - CH-6500 Bellinzona
contact email	matteo.pecoraro@irb.usi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD040957

PRIDE project URI

• PRIDE
  1. PXD040957
     1. Label: PRIDE project
     2. Name: Proteomics of immune cells from liver tumors reveals immunotherapy targets