PXD040915-2

PXD040915 is an original dataset announced via ProteomeXchange.

Title	Cytochrome c Lysine Acetylation Regulates Cellular Respiration and Cell Death in Ischemic Skeletal Muscle
Description	Cytochrome c (Cytc) is essential for mitochondrial respiration and apoptosis. Tissue specific post-translational modifications of Cytc play an important regulatory role in these processes. Here, we describe a new acetylation site, lysine 39, which was mapped in ischemic porcine skeletal muscle. Recombinant acetylmimetic K39Q protein demonstrated increased cytochrome c oxidase (COX) activity and decreased caspase-3 and cardiolipin peroxidase activities. Cytc double knockout cells expressing acetylmimetic K39Q Cytc showed an increase in mitochondrial respiration, mitochondrial membrane potential, and mitochondrial ROS production and a decrease in cell death. These results are discussed in the context of X-ray crystallography structures of K39 acetylated (1.50 Å) and acetylmimetic K39Q Cytc (1.36 Å) and NMR dynamics. We propose that lysine 39 acetylation is an adaptive response allowing skeletal muscle to meet heightened energy demand while simultaneously providing the tissue with robust resilience to ischemia-reperfusion injury.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:52:38.111.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040915
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Meierhofer
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-16 07:26:30	ID requested
1	2023-07-10 07:52:18	announced
⏵ 2	2024-10-22 05:52:38	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Acetylation, cytochrome C, ischimic muscle

Maik Hüttemann
contact affiliation	Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI 48201, USA
contact email	mhuttema@med.wayne.edu
lab head
David Meierhofer
contact affiliation	Mass Spectrometry Facility MPIMG
contact email	meierhof@molgen.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040915
     1. Label: PRIDE project
     2. Name: Cytochrome c Lysine Acetylation Regulates Cellular Respiration and Cell Death in Ischemic Skeletal Muscle