PXD040912-1

PXD040912 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteomics reveal novel roles of cAMP in plants
Description	3′,5′-cyclic adenosine monophosphate (cAMP) is finally recognized as an essential signaling molecule in plants where cAMP-dependent processes include responses to hormones and environmental stimuli. To better understand the role of cAMP at the systems level, we have undertaken a phosphoproteomic analysis to elucidate the cAMP-dependent response of tobacco BY-2 cells. These cells overexpress a “sponge” that buffers intracellular cAMP level. The results show that, firstly, in vivo cAMP dampening profoundly affects the plant kinome and notably mitogen-activated protein kinases, receptor-like kinases and calcium-dependent protein kinases, thereby modulating the cellular responses at the systems level. Secondly, buffering cAMP levels also affects mRNA processing through the modulation of the phosphorylation status of several RNA-binding proteins with roles in splicing, including many SR proteins. Thirdly, cAMP-dependent phosphorylation targets appear to be conserved among plant species. Taken together, these findings are consistent with an ancient role of cAMP in mRNA processing and cellular programming and suggest that unperturbed cellular cAMP levels are essential for cellular homeostasis and signaling in plant cells.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_01:09:12.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Milena Marsoni
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-16 02:12:47	ID requested
⏵ 1	2024-05-24 01:09:13	announced

Domingo G, Marsoni M, Davide E, Fortunato S, de Pinto MC, Bracale M, Molla G, Gehring C, Vannini C, The cAMP-dependent phosphorylation footprint in response to heat stress. Plant Cell Rep, 43(6):137(2024) [pubmed]

10.1007/s00299-024-03213-y;

submitter keyword: cyclic nucleotide, cAMP,cyclic adenosine monophosphate, RNA splicing, kinases

Guido Domingo
contact affiliation	University of Insubria
contact email	g.domingo@uninsubria.it
lab head
Milena Marsoni
contact affiliation	University of Insubria
contact email	milena.marsoni@uninsubria.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040912
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteomics reveal novel roles of cAMP in plants