PXD040898 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A combined gas phase separation strategy for ADP-ribosylated peptides |
Description | ADP-ribosylation (ADPr) is a posttranslational modification that is best studied using mass spectrometry. Method developments that are permissive to low inputs or baseline levels of protein ribosylation represent the next frontier in the field. High-field asymmetric waveform ion mobility spectrometry (FAIMS) reduces peptide complexity in gas phase, providing a means to achieve maximal ADPr peptide sequencing depth. We therefore investigated the extent to which FAIMS with or without traditional gas-phase fractionation/separation (GPS) can increase the number of ADPr peptides. We examined ADPr peptides enriched from mouse spleens. We gleaned additional insight by also reporting findings from the corresponding non-ADPr peptide contaminants, and the peptide inputs for ADPr peptide enrichment. At increasingly higher compensation voltages, ADPr peptides were more stable whereas the non-ADPr peptides were filtered out. A combination of 3 GPS survey scans, each with 8 compensation voltages resulted in 790 high-confidence ADPr peptides, compared to 90 with GPS alone. A simplified acquisition strategy requiring only two injections corresponding to two MS1 scan ranges coupled to optimized compensation voltage settings, provided 402 ADPr peptides corresponding to 234 ADPr proteins. We conclude that our combined gas phase separation strategy is a valuable addition to any ADP-ribosylome workflow. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:04:56.139.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD040898 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Sasha Singh |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | adenosine diphosphoribosyl (ADP-ribosyl) modified residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-15 12:47:53 | ID requested | |
1 | 2023-08-30 16:04:23 | announced | |
⏵ 2 | 2023-11-14 09:05:04 | announced | 2023-11-14: Updated project metadata. |
Publication List
Kasai T, Kuraoka S, Higashi H, Delanghe B, Aikawa M, Singh SA, A Combined Gas-Phase Separation Strategy for ADP-ribosylated Peptides. J Am Soc Mass Spectrom, 34(10):2136-2145(2023) [pubmed] |
10.6019/PXD040898; |
Keyword List
submitter keyword: mono-ADP-ribose,Gas-phase segmentation, ribosylation, high field asymmetric waveform ion mobility spectrometry, gas-phase fragmentation |
Contact List
Masanori Aikawa |
contact affiliation | Brigham and Women's Hospital |
contact email | maikawa@bwh.harvard.edu |
lab head | |
Sasha Singh |
contact affiliation | Brigham and Women's Hospital, Harvard Medical School |
contact email | sasingh@bwh.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040898
- Label: PRIDE project
- Name: A combined gas phase separation strategy for ADP-ribosylated peptides