PXD040897-2

PXD040897 is an original dataset announced via ProteomeXchange.

Title	Quantitative, TMT-based proteomics and phosphoproteomics analysis of SARS-COV-2 infected cells.
Description	Host kinases play essential roles in the host cell cycle, innate immune signaling, the stress response to viral infection, and inflammation. Previous work has demonstrated coronaviruses specifically target kinase cascades to subvert host cell responses to infection and rely upon host kinase activity to phosphorylate viral proteins to enhance replication. Given the number of kinase inhibitors that are already FDA approved to treat cancers, fibrosis, and other human disease, they represent an attractive class of compounds to repurpose for host targeted therapies against emerging coronavirus infections. To further understand the host kinome response to betacoronavirus infection we employed multiplex inhibitory bead mass spectrometry (MIB-MS) following MERS-CoV and SARS-CoV-2 infection of human lung epithelial cell lines. To determine signaling pathways altered over the SARS-CoV-2 time-course, global quantitative phosphoproteomic and proteomic analysis of SARS-CoV-2 infected A549-hACE2 was performed on paired cell lysates (n=3) from the MIB-MS analysis. This PRIDE Submission contains the proteome and phosphoproteome data. The MIB-MS data were uploaded as a separate PRIDE Submission. Over 7,200 proteins and 14,300 phosphosites were quantified over the 24 hr infection time course. Further analysis of the phosphoproteome indicated activation of MAPK, PI3K, and mTOR signaling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:33.200.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angie Mordant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-15 11:47:46	ID requested
1	2023-08-01 13:28:54	announced
⏵ 2	2024-10-22 05:59:33	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Proteome

Phosphoproteome

Coronavirus

SARS-COV-2

Labelled Quantitation

TMT labelling

TMTpro 16plex

LC-MS/MS.

Ralph Baric
contact affiliation	1. Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. 2. Department of Epidemiology, Gillings School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. 3. Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
contact email	rbaric@email.unc.edu
lab head
Angie Mordant
contact affiliation	UNC Proteomics Core, Department of Pharmacology, University of North Carolina at Chapel Hill
contact email	angie_mordant@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD040897

PRIDE project URI

• PRIDE
  1. PXD040897
     1. Label: PRIDE project
     2. Name: Quantitative, TMT-based proteomics and phosphoproteomics analysis of SARS-COV-2 infected cells.