PXD040896 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Monitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry |
Description | We propose here a workflow based on an automated immunocapture followed by top-down and middle-down LC-MS/MS approaches to characterize mAb proteoforms spiked in mouse plasma. We demonstrate the applicability of our workflow on a large concentration range using pembrolizumab as a model. We also compare the performance of two state-of-the-art Orbitrap platforms (Tribrid Eclipse and Exploris 480) for these studies. The added value of our workflow for an accurate and sensitive characterization of mAb proteoforms in mouse plasma is highlighted. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-27 |
AnnouncementXML | Submission_2024-08-27_08:24:36.085.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jonathan Dhenin |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | deamidated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-15 11:41:46 | ID requested | |
⏵ 1 | 2024-08-27 08:24:36 | announced | |
Publication List
Dhenin J, Lafont V, Dupr, é M, Krick A, Mauriac C, Chamot-Rooke J, Monitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry. Proteomics, 24(3-4):e2300069(2024) [pubmed] |
10.1002/pmic.202300069; |
Keyword List
submitter keyword: immunocapture, monoclonal antibody, proteoforms, middle-down mass spectrometry, top-down mass spectrometry |
Contact List
Julia Chamot-Rooke |
contact affiliation | Institut Pasteur, Université Paris Cité, CNRS UAR2024, Mass Spectrometry for Biology, Paris, 75015, France |
contact email | julia.chamot-rooke@pasteur.fr |
lab head | |
Jonathan Dhenin |
contact affiliation | DMPK, Sanofi R&D |
contact email | jonathan.dhenin@sanofi.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD040896 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040896
- Label: PRIDE project
- Name: Monitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry