PXD040896-1

PXD040896 is an original dataset announced via ProteomeXchange.

Title	Monitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry
Description	We propose here a workflow based on an automated immunocapture followed by top-down and middle-down LC-MS/MS approaches to characterize mAb proteoforms spiked in mouse plasma. We demonstrate the applicability of our workflow on a large concentration range using pembrolizumab as a model. We also compare the performance of two state-of-the-art Orbitrap platforms (Tribrid Eclipse and Exploris 480) for these studies. The added value of our workflow for an accurate and sensitive characterization of mAb proteoforms in mouse plasma is highlighted.
HostingRepository	PRIDE
AnnounceDate	2024-08-27
AnnouncementXML	Submission_2024-08-27_08:24:36.085.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Dhenin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-15 11:41:46	ID requested
⏵ 1	2024-08-27 08:24:36	announced

Dhenin J, Lafont V, Dupr, é M, Krick A, Mauriac C, Chamot-Rooke J, Monitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry. Proteomics, 24(3-4):e2300069(2024) [pubmed]

10.1002/pmic.202300069;

submitter keyword: immunocapture, monoclonal antibody, proteoforms, middle-down mass spectrometry, top-down mass spectrometry

Julia Chamot-Rooke
contact affiliation	Institut Pasteur, Université Paris Cité, CNRS UAR2024, Mass Spectrometry for Biology, Paris, 75015, France
contact email	julia.chamot-rooke@pasteur.fr
lab head
Jonathan Dhenin
contact affiliation	DMPK, Sanofi R&D
contact email	jonathan.dhenin@sanofi.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD040896

PRIDE project URI

• PRIDE
  1. PXD040896
     1. Label: PRIDE project
     2. Name: Monitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry