PXD040870-2

PXD040870 is an original dataset announced via ProteomeXchange.

Title	Translational and post-translational dynamics in a model peptidergic system
Description	The cell bodies of hypothalamic magnocellular neurones are densely packed in the hypothalamic supraoptic nucleus whereas their axons project to the anatomically discrete posterior pituitary gland. We have taken advantage of this unique anatomical structure to establish proteome and phosphoproteome dynamics in neuronal cell bodies and axonal terminals in response to physiological stimulation. We have found that proteome and phosphoproteome responses to neuronal stimulation are very different between somatic and axonal neuronal compartments, indicating the need of each cell domain to differentially adapt. In particular, changes in the phosphoproteome in the cell body are involved in the reorganisation of the cytoskeleton and in axonal terminals the regulation of synaptic and secretory processes. We have identified that prohormone precursors including vasopressin and oxytocin are phosphorylated in axonal terminals and are hyperphosphorylated following stimulation. By multi-omic integration of transcriptome and proteomic data we identify changes to proteins present in afferent inputs to this nucleus.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:14.656.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Gillard
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-14 14:07:24	ID requested
1	2023-04-12 02:16:14	announced
⏵ 2	2023-11-14 08:45:15	announced	2023-11-14: Updated project metadata.

B, á, rez-L, ó, pez S, Mecawi AS, Bryan N, Pau, ž, a AG, Duque VJ, Gillard BT, Murphy D, Greenwood MP, Translational and Posttranslational Dynamics in a Model Peptidergic System. Mol Cell Proteomics, 22(5):100544(2023) [pubmed]

submitter keyword: cytoskeleton, phosphoproteome,Posterior Pituitary, Supraoptic nucleus, proteome

David Murphy
contact affiliation	Translational Health Sciences, Bristol Medical School, Faculty of Health Sciences, University of Bristol
contact email	david.murphy@bristol.ac.uk
lab head
Benjamin Gillard
contact affiliation	University of Bristol
contact email	bg14337@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD040870

PRIDE project URI

• PRIDE
  1. PXD040870
     1. Label: PRIDE project
     2. Name: Translational and post-translational dynamics in a model peptidergic system