PXD040868-1

PXD040868 is an original dataset announced via ProteomeXchange.

Title	Resolving the temporal and spatial splenic proteome during fungal infection for putative dual perspective biomarker discovery
Description	Fungal pathogens are emerging threats to global health and the rise of incidence is associated with climate change and increased geographical distribution; factors also influencing host susceptibility to infection. Accurate detection and diagnosis of fungal infections is paramount to align options for rapid and effective therapeutic treatments. For improved diagnostics, the discovery and development of biomarkers presents a promising avenue; however, this approach requires a priori knowledge of markers of infection. To uncover putative novel biomarkers of disease, profiling of the host immune response and pathogen virulence factor production is indispensable. In this study, we use mass spectrometry-based proteomics to resolve the temporal and spatial proteomes of Cryptococcus neoformans infection of the spleen following a murine model of infection. Dual perspective proteome profiling defines global remodeling of the host over a time course of infection, confirming activation of immune associated proteins connected to fungal infections. Conversely, pathogen proteomes detect well-characterized C. neoformans virulence determinants, along with novel mapped patterns of pathogenesis during progression of disease. Together, our systematic approach confirms immune protection against fungal pathogens and explores the discovery of putative biomarkers from complementary biological systems for monitoring the presence and progression of cryptococcal disease.
HostingRepository	PRIDE
AnnounceDate	2023-05-23
AnnouncementXML	Submission_2023-05-23_13:29:27.324.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BenjaminMuselius
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-14 12:16:13	ID requested
⏵ 1	2023-05-23 13:29:27	announced
2	2023-11-14 08:59:35	announced	2023-11-14: Updated project metadata.

submitter keyword: Cryptococcus neoformans, spatial immune response, biomarkers,Mass spectrometry-based proteomics, in vivo murine model, temporal immune response, spleen

JenniferGeddes-McAlister
contact affiliation	University of Guelph, Ontario, Canada
contact email	jgeddesm@uoguelph.ca
lab head
BenjaminMuselius
contact affiliation	University of Guelph
contact email	bmuseliu@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040868
     1. Label: PRIDE project
     2. Name: Resolving the temporal and spatial splenic proteome during fungal infection for putative dual perspective biomarker discovery