PXD040824-1

PXD040824 is an original dataset announced via ProteomeXchange.

Title	Subcellular proteomics for determining iron limited remodeling of plastids in the model diatom Thalassiosira pseudonana
Description	Diatoms are important primary producers in the world’s oceans, yet their growth is constrained in large regions by low bioavailable iron (Fe). Low Fe-induced limitation of primary production is due to requirements for Fe in components of essential metabolic pathways including photosynthesis and other chloroplast plastid functions. Studies have shown that under Fe-limited stress, diatoms alter plastid-specific processes, including components of electron transport. These physiological changes suggest changes of protein content and their abundance within the diatom plastid. While in-silico predictions provide putative information on plastid-localized proteins, knowledge of diatom plastid proteins remains limited in comparison to model photosynthetic organisms. To characterize proteins enriched in diatom plastids we have used shotgun proteomics to assess the proteome of subcellular plastid-enriched fractions from Thalassiosira pseudonana. To improve our understanding of how the plastid proteome is remodeled in response to Fe limitation, proteome sequencing has been performed on T. pseudonana grown under Fe replete and limited conditions. These analyses have shown that Fe limitation regulates major metabolic pathways in the plastid, including the Calvin cycle, as well as changes in light harvesting protein expression. In-silico localization predictions of proteins identified in this plastid-enriched proteome allowed for an in-depth comparison of theoretical vs observed plastid-localization, providing evidence for the potential of additional protein import pathways into the diatom plastid.
HostingRepository	PRIDE
AnnounceDate	2023-08-04
AnnouncementXML	Submission_2023-08-04_10:08:39.503.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BrookNunn
SpeciesList	scientific name: Thalassiosira pseudonana CCMP1335; NCBI TaxID: 296543;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-13 11:01:04	ID requested
⏵ 1	2023-08-04 10:08:39	announced
2	2024-10-22 05:59:50	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: plastid, organelle, iron limitation, diatom, ocean

BrookNunn
contact affiliation	University of Washington Department of Genome Sciences
contact email	brookh@uw.edu
lab head
BrookNunn
contact affiliation	University of Washington
contact email	brookh@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040824
     1. Label: PRIDE project
     2. Name: Subcellular proteomics for determining iron limited remodeling of plastids in the model diatom Thalassiosira pseudonana