PXD040806 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC |
Description | Hitherto, most studies on POLD1 have mainly focused on the effect of POLD1 inactivation mutation in tumors. Nonetheless, the mechanism underlying high POLD1 expression in tumorigenesis remains elusive. Herein, we substantiated the pro-carcinogenic role of POLD1 in bladder cancer (BLCA) and found that POLD1 expression is related to malignancy and prognosis of BLCA. Next, we demonstrated that POLD1 could promote proliferation and metastasis of BLCA via MYC. Mechanistically, we demonstrated that POLD1 was able to stabilize MYC in a manner independent of DNA polymerase activity. POLD1 attenuated the FBXW7-mediated ubiquitination degradation of MYC by directly binding to the MYC homology box 1 domain competitively with FBXW7. Moreover, we found that POLD1 can form a complex with MYC to promote the transcriptional activity of MYC. MYC could also transcriptionally activate POLD1, forming a POLD1-MYC positive feedback loop to enhance the pro-carcinogenic effect of POLD1-MYC on BLCA. Overall, our study suggests a novel MYC-driven mechanism for BLCA, and POLD1 has the potential as a biomarker for BLCA. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-29 |
AnnouncementXML | Submission_2023-03-28_22:11:29.003.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | YejinpengWang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-13 05:47:26 | ID requested | |
⏵ 1 | 2023-03-28 22:11:29 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: DNA polymerase δ (POLD1) |
Bladder cancer (BLCA) |
MYC |
FBXW7 |
Ubiquitination degradation. |
Contact List
YuXiao |
contact affiliation | Wuhan University |
contact email | yu.xiao@whu.edu.cn |
lab head | |
YejinpengWang |
contact affiliation | Wuhan University |
contact email | yejinpeng.wang@whu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD040806 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040806
- Label: PRIDE project
- Name: DNA polymerase POLD1 promotes proliferation and metastasis of bladder cancer by stabilizing MYC