PXD040787-2

PXD040787 is an original dataset announced via ProteomeXchange.

Title	comparative phosphoproteomics uncover proteins from the mechanobiome in Entamoeba histolytica
Description	Entamoeba histolytica is a protozoan parasite and the causative agent of amoebiasis in humans. This amoeba invades human tissues by taking advantage of the actin-rich cytoskeleton to move, enter the tissue matrix, kill and phagocyte human cells. During events leading to tissue invasion, E. histolytica moves from the intestinal lumen to cross the mucus layer and enter the epithelial parenchyma. Faced with the chemical and physical constraints of these diverse environments, E. histolytica has developed sophisticated systems to integrate internal and external signals and to coordinate cell shape changes and motility. These cell signalling circuits are driven by the interactions between the parasite and the extracellular matrix combined with rapid responses from the mechanobiome protein network in which protein phosphorylation is important. To understand phosphorylation events we focus on phosphatidylinositol 3-kinases and we have performed live cell imaging and phosphoproteomics. The results highlight 1150 proteins within the amoebic proteome being members of the phosphoproteome, including signalling and structural molecules involved in cytoskeleton activities. Inhibition of phosphatidylinositol 3-kinases alters phosphorylation dynamics in important members of these categories, a finding that correlates with changes in the kinetic parameters of amoeba motility and morphology, as well as with altered content of actin-rich adhesive structures
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:20.766.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	gagan jhingan
SpeciesList	scientific name: Entamoeba histolytica; NCBI TaxID: NCBITaxon:5759; scientific name: Entamoeba histolytica; NCBI TaxID: 5759;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-11 06:19:59	ID requested
1	2023-05-15 13:04:25	announced
⏵ 2	2023-11-14 09:00:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: entamoeba, wortmannin, phosphoproteomics

Nancy Guillen
contact affiliation	Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris cedex 15, France
contact email	gaganjhingan@hotmail.com
lab head
gagan jhingan
contact affiliation	VProteomics
contact email	gaganjhingan@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040787
     1. Label: PRIDE project
     2. Name: comparative phosphoproteomics uncover proteins from the mechanobiome in Entamoeba histolytica