PXD040785-1

PXD040785 is an original dataset announced via ProteomeXchange.

Title	Myocardial proteome changes in aortic stenosis rats subjected to long-term aerobic exercise
Description	The effects of exercise training (ET) on the heart of aortic stenosis (AS) rats are controversial and the mechanisms involved in alterations induced by ET have been poorly clarified. In this study we analyzed the myocardial proteome to identify proteins modulated by moderate intensity aerobic ET in rats with chronic supravalvar AS. Wistar rats were divided into four groups: sedentary control (C-Sed), exercised control (C-Ex), sedentary aortic stenosis (AS-Sed), and exercised AS (AS-Ex). ET consisted of five treadmill running sessions per week for 16 weeks. Statistical analysis was performed by ANOVA or Kruskal-Wallis and Goodman tests. Results were discussed at a significance level of 5%. At the end of the experiment, AS-Ex rats had higher functional capacity, lower blood lactate concentration, and better cardiac structural and left ventricular functional parameters than the AS-Sed. Myocardial proteome analysis showed that AS-Sed had higher protein expressions related to the glycolytic pathway, oxidative stress, and inflammation, and lower protein expressions related to beta-oxidation than C-Sed. AS-Ex had higher expression of one protein related to mitochondrial biogenesis and lower protein expressions associated with oxidative stress and inflammation than AS-Sed. Proteomic data were validated for proteins related to lipid and glycolytic metabolism. In conclusion, chronic pressure overload changes the expression of myocardial proteins that are mainly involved in lipid and glycolytic energy metabolism in rats. Moderate intensity aerobic training attenuates protein expressions related to oxidative stress and inflammation and increases protein expressions related to mitochondrial biogenesis. Protein changes are associated with improved functional capacity, cardiac remodeling and left ventricular function in aortic stenosis rats.
HostingRepository	PRIDE
AnnounceDate	2024-02-29
AnnouncementXML	Submission_2024-02-29_08:21:21.326.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Licia C. Silva-Costa
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Xevo Q-Tof

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-10 21:21:30	ID requested
⏵ 1	2024-02-29 08:21:21	announced

10.1002/JCP.31199;

submitter keyword: supravalvular aortic stenosis, myocardium, ventricular dysfunction, rat, aerobic exercise training,Proteomics

Gustavo Augusto
contact affiliation	Department of Internal Medicine, Botucatu Medical School, São Paulo State University, UNESP, SP, Brazil
contact email	gustavo.mota@unesp.br
lab head
Licia C. Silva-Costa
contact affiliation	Unicamp
contact email	l065387@g.unicamp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040785
     1. Label: PRIDE project
     2. Name: Myocardial proteome changes in aortic stenosis rats subjected to long-term aerobic exercise