PXD040781 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative, quantitative proteomic analysis of cell shape transitions in Haloferax volcanii |
Description | Cell shape plays a crucial role in microbial survival. While Haloferax volcanii, a model haloarchaeon, forms rods and disks, depending on environmental conditions, little is known about mechanisms underpinning archaeal cell-shape determination. We identified mutants that exclusively form rods and carried out comprehensive proteomics studies in which we compared these mutants to previously identified disk-only mutant strains and wild-type. Using this approach, we identified several additional candidates for shape determination. The generation of deletion mutants lacking genes encoding potential rod- and disk-determining factors, HVO_2174 (RdfA) and HVO_2176 (DdfA), respectively, resulted in rod- and disk-defective phenotypes. Comprehensive proteomics performed with ∆rdfA and ∆ddfA on these shape mutants implicated a diverse set of proteins, including transporters, signaling components, and transducers, as important for cell shape determination. We also identified structural proteins including the H. volcanii actin homolog volactin (VolA), a previously unknown cytoskeletal element, required for disk-shape morphogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-17 |
AnnouncementXML | Submission_2024-02-17_11:43:42.595.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Schulze |
SpeciesList | scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246; scientific name: Archaea; NCBI TaxID: NCBITaxon:2157; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse; Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-10 16:03:50 | ID requested | |
⏵ 1 | 2024-02-17 11:43:43 | announced | |
Publication List
10.1038/s41467-024-45196-0; |
Schiller H, Hong Y, Kouassi J, Rados T, Kwak J, DiLucido A, Safer D, Marchfelder A, Pfeiffer F, Bisson A, Schulze S, Pohlschroder M, Identification of structural and regulatory cell-shape determinants in Haloferax volcanii. Nat Commun, 15(1):1414(2024) [pubmed] |
Keyword List
submitter keyword: Archaeal Proteome Project, Ursgal,Archaea, Haloferax volcanii, label-free quantification, cell shape |
Contact List
Mechthild Pohlschroder |
contact affiliation | University of Pennsylvania, Department of Biology |
contact email | pohlschr@sas.upenn.edu |
lab head | |
Stefan Schulze |
contact affiliation | University of Pennsylvania |
contact email | sschulze@sas.upenn.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040781
- Label: PRIDE project
- Name: Comparative, quantitative proteomic analysis of cell shape transitions in Haloferax volcanii