PXD040777-2

PXD040777 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomics reveals disruption of metal homeostasis and metalloproteins by the antibiotic holomycin
Description	The natural product holomycin contains a unique cyclic disulfide and exhibits broad-spectrum antimicrobial activities. Reduced holomycin chelates metal ions with high affinity and disrupts metal homeostasis in the cell. To identify cellular metalloproteins that are affected by holomycin, reactive-cysteine profiling was performed using isotopic Tandem Orthogonal Proteolysis–Activity-based Protein Profiling. This chemoproteomic analysis showed that holomycin treatment increases the reactivity of metal-coordinating cysteine residues in several zinc-dependent and iron-sulfur cluster-dependent enzymes. Whole-proteome abundance analysis revealed that holomycin treatment induces zinc starvation, iron starvation, and cellular stress. This study sets the stage for investigating the impact of metal-binding molecules on metalloproteomes using chemoproteomics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:00:20.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Falco
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	dimethylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Discovery; LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-10 09:33:30	ID requested
1	2024-10-08 11:21:45	announced
⏵ 2	2024-10-22 06:00:21	announced	2024-10-22: Updated project metadata.

10.1021/ACSCHEMBIO.3C00360;

submitter keyword: antibiotics,chemoproteomics, metalloproteins

Eranthie Weerapana
contact affiliation	Boston College Department of Chemistry
contact email	Eranthie@bc.edu
lab head
Julia Falco
contact affiliation	Boston College
contact email	falcoju@bc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040777
     1. Label: PRIDE project
     2. Name: Chemoproteomics reveals disruption of metal homeostasis and metalloproteins by the antibiotic holomycin