PXD040760 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Perinuclear Vimentin-RNF26 contacts integrate ER and endosomal responses to ER stress |
Description | Compartmentalization of organelles in space and time affects their functional state and enables higher order regulation of essential cellular processes. How organellar residence is maintained in a defined area of the cell remains poorly understood. In this study, we uncover a new role for intermediate filaments in the maintenance of organellar architecture and dynamics, which is executed through a direct connection between Vimentin and the ER-embedded ubiquitin ligase ring finger protein 26 (RNF26). While the ubiquitin ligase function of RNF26 promotes perinuclear positioning of endolysosomes through binding endosomal adapters, catalytically inactive RNF26 preferentially binds Vimentin through a C-terminal motif. Loss of either RNF26 or Vimentin redistributes endolysosomes and ER membranes from the perinuclear ER towards the periphery. Furthermore, RNF26 and Vimentin control changes in ER morphology and invoke organelle compartmentalization during ER stress with consequences for stress-associated ER-phagy. Collectively, we define a new function for Vimentin-containing intermediate filaments as anchors of a dynamic interplay between the ER and endosomes, critical to the integrity of the perinuclear ER and corresponding perinuclear endosomal cloud during homeostatic and stress conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:04:57.569.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rayman Tjokrodirijo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ FT Ultra |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-10 02:04:01 | ID requested | |
1 | 2023-09-05 08:07:07 | announced | |
⏵ 2 | 2023-11-14 09:04:58 | announced | 2023-11-14: Updated project metadata. |
Publication List
Cremer T, Voortman LM, Bos E, Jongsma ML, Ter Haar LR, Akkermans JJ, Talavera Orme, ñ, o CM, Wijdeven RH, de Vries J, Kim RQ, Janssen GM, van Veelen PA, Koning RI, Neefjes J, Berlin I, RNF26 binds perinuclear vimentin filaments to integrate ER and endolysosomal responses to proteotoxic stress. EMBO J, 42(18):e111252(2023) [pubmed] |
Keyword List
submitter keyword: Vimentin, ER stress, endosomes, intermediate filaments, RNF26 |
Contact List
Peter A. van Veelen |
contact affiliation | Head Proteomics Group, Leiden University Medical Center, Center for Proteomics and Metabolomics, PO Box 9600, Postal zone P1-Q, 2300 RC Leiden, The Netherlands |
contact email | P.A.van_Veelen@lumc.nl |
lab head | |
Rayman Tjokrodirijo |
contact affiliation | Leiden University Medical Center, Proteomics group |
contact email | R.T.N.Tjokrodirijo@lumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040760
- Label: PRIDE project
- Name: Perinuclear Vimentin-RNF26 contacts integrate ER and endosomal responses to ER stress