PXD040737 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A solid-phase compatible silane-based cleavable linker enables custom isobaric quantitative chemoproteomics |
Description | Only ~4% of the human proteome has been drugged by FDA approved molecules. Consequently, closing this druggability gap is a central focus of mass spectrometry chemoproteomics screening platforms. Despite increasingly widespread adoption, established chemoproteomic platforms fall short of achieving comprehensive proteome-wide structure activity relationship (SAR) maps for two key reasons: (1) time consuming and cumbersome sample preparation workflows and (2) and low throughput sample acquisition. Here we report the silane-based Cleavable Linkers for Isotopically-labeled Proteomics (sCLIP) method. sCLIP streamlines sample preparation with unparalleled early-stage isobaric labeling and sample pooling, allowing for increased sample throughput via customized low cost 6-plex sample multiplexing. The sCLIP method is distinguished by its unprecedented click-assembled isobaric tags, in which the reporter group is encoded in the sCLIP capture reagent and balancer in the pan cysteine-reactive probe. When paired with a custom FragPipe data analysis workflow and applied to cysteine-reactive fragment screens, sCLIP proteomics revealed established and unprecedented cysteine-ligand pairs, including those labeled by covalent-reversible electrophilic modalities. |
HostingRepository | PRIDE |
AnnounceDate | 2023-09-20 |
AnnouncementXML | Submission_2023-09-20_11:32:11.461.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | NikBurton |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-03-09 15:02:54 | ID requested | |
⏵ 1 | 2023-09-20 11:32:11 | announced | |
2 | 2023-11-14 09:04:49 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: cleavable, MS2, TMT, isobaric, sCLIP, DADPS,LC-MSMS, solid-phase |
Contact List
Keriann MarieBackus |
contact affiliation | Department of Biological Chemistry, University of California Los Angeles, Los Angeles, California |
contact email | kbackus@mednet.ucla.edu |
lab head | |
NikBurton |
contact affiliation | UCLA |
contact email | nrburton@ucla.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040737
- Label: PRIDE project
- Name: A solid-phase compatible silane-based cleavable linker enables custom isobaric quantitative chemoproteomics