PXD040696-2

PXD040696 is an original dataset announced via ProteomeXchange.

Title	A solid-phase compatible silane-based cleavable linker enables custom isobaric quantitative chemoproteomics
Description	Only ~4% of the human proteome has been drugged by FDA approved molecules. Consequently, closing this druggability gap is a central focus of mass spectrometry chemoproteomics screening platforms. Despite increasingly widespread adoption, established chemoproteomic platforms fall short of achieving comprehensive proteome-wide structure activity relationship (SAR) maps for two key reasons: (1) time consuming and cumbersome sample preparation workflows and (2) and low throughput sample acquisition. Here we report the silane-based Cleavable Linkers for Isotopically-labeled Proteomics (sCLIP) method. sCLIP streamlines sample preparation with unparalleled early-stage isobaric labeling and sample pooling, allowing for increased sample throughput via customized low cost 6-plex sample multiplexing. The sCLIP method is distinguished by its unprecedented click-assembled isobaric tags, in which the reporter group is encoded in the sCLIP capture reagent and balancer in the pan cysteine-reactive probe. When paired with a custom FragPipe data analysis workflow and applied to cysteine-reactive fragment screens, sCLIP proteomics revealed established and unprecedented cysteine-ligand pairs, including those labeled by covalent-reversible electrophilic modalities.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:39:26.094.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nik Burton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-08 17:05:33	ID requested
1	2023-09-20 12:22:09	announced
⏵ 2	2023-11-14 06:39:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: cleavable, MS2, TMT, isobaric, sCLIP, DADPS,LC-MSMS, solid-phase

Keriann Marie Backus
contact affiliation	Department of Biological Chemistry, University of California Los Angeles, Los Angeles, California
contact email	Kbackus@mednet.ucla.edu
lab head
Nik Burton
contact affiliation	UCLA
contact email	nrburton@ucla.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD040696

PRIDE project URI

• PRIDE
  1. PXD040696
     1. Label: PRIDE project
     2. Name: A solid-phase compatible silane-based cleavable linker enables custom isobaric quantitative chemoproteomics