PXD040635-1

PXD040635 is an original dataset announced via ProteomeXchange.

Title	SPARKEL depletion time course proteome
Description	Apicomplexan parasites are ubiquitous pathogens of humans. Toxoplasma gondii, a model apicomplexan, possesses a divergent ortholog of mammalian PDK1. This ortholog, TgSPARK, is essential for the ability of parasites to invade and exit their host. We discovered an interactor of SPARK, an elogin-like protein that we termed SPARKEL. Here, we conduct a quantitative mass spectrometry experiment to measure changes to the parasite proteome following 0, 3, 8, and 24 hours of conditional SPARKEL depletion.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:36:03.939.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040635
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alice Herneisen
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-06 13:55:29	ID requested
⏵ 1	2024-10-08 11:36:04	announced
2	2024-10-22 06:49:37	announced	2024-10-22: Updated project metadata.

10.6019/PXD040635;

10.7554/ELIFE.93877.1;

submitter keyword: Toxoplasma gondii, parasite

Sebastian Lourido
contact affiliation	Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology
contact email	lourido@wi.mit.edu
lab head
Alice Herneisen
contact affiliation	Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology
contact email	alice@wi.mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD040635

PRIDE project URI

• PRIDE
  1. PXD040635
     1. Label: PRIDE project
     2. Name: SPARKEL depletion time course proteome