PXD040585-1

PXD040585 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	DiLeu isobaric labeling coupled with limited proteolysis mass spectrometry for high-throughput profiling of protein structural changes in Alzheimers disease
Description	High-throughput quantitative analysis of protein conformational changes has a profound impact on our understanding of the pathological mechanisms of Alzheimers disease (AD). To establish an effective workflow enabling quantitative analysis of changes in protein conformation within multiple samples simultaneously, here we report the combination of N,N-dimethyl leucine (DiLeu) isobaric tag labeling with limited-proteolysis mass spectrometry (DiLeu-LiP-MS) for high-throughput structural protein quantitation in serum samples collected from AD patients and control donors. 23 proteins were discovered to undergo structural changes, mapping to 35 unique conformotypic peptides with significant changes between the AD group and the control group. 7 out of 23 proteins, including CO3, CO9, C4BPA, APOA1, APOA4, C1R and APOA, exhibited potential correlation with AD. Moreover, we found that complement proteins (e.g., CO3, CO9 and C4BPA) related to AD exhibited elevated levels in the AD group compared to those in the control group. These results provide evidence that the established DiLeu-LiP-MS method can be used for high-throughput structural protein quantitation, which also showed great potential in achieving large-scale and in-depth quantitative analysis of protein conformational changes in other biological systems.
HostingRepository	MassIVE
AnnounceDate	2025-01-28
AnnouncementXML	Submission_2025-01-28_09:08:13.800.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haiyan Lu
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-03 12:33:00	ID requested
⏵ 1	2025-01-28 09:08:14	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: DiLeu isobaric labeling, limited proteolysis mass spectrometry, high-throughput protein quantitation, structural protein quantitation, Alzheimers disease

submitter keyword: DiLeu isobaric labeling, limited proteolysis mass spectrometry, high-throughput protein quantitation, structural protein quantitation, Alzheimers disease

Contact List

Lingjun Li
contact affiliation	University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Haiyan Lu
contact affiliation	University of Wisconsin Madison
contact email	hlu244@wisc.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091408/