PXD040540-1
PXD040540 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Deep Coverage of Arginine Phosphorylation Enriched in Metabolism Pathways of Mycolicibacterium smegmatis |
| Description | Protein phosphorylation is one of the major posttranslational modifications that regulates protein expression and activity in bacteria. Although serine, threonine and tyrosine phosphorylation have been well-established, arginine phosphorylation has gained attention in recent decades due to its global importance in processes such as protein degradation, competence, stringent response, and other stress response. Mycolicibacterium smegmatis, a nonpathogenic surrogate of Mycobacterium tuberculosis, is a predominate model for studying the basic biology of mycobacterial pathogenesis. A recent study confirmed the presence of arginine phosphorylation in M. smegmatis and identified six arginine phosphorylated proteins based on shotgun proteomics analysis. Here, to provide a more comprehensive understanding, we performed a global, in-depth analysis of the M. smegmatis arginine phosphoproteome using high-accuracy mass spectrometry in combination with an optimized titanium-immobilized metal ion affinity chromatography (Ti4+-IMAC) phosphopeptide enrichment strategy. |
| HostingRepository | iProX |
| AnnounceDate | 2023-03-01 |
| AnnouncementXML | Submission_2023-03-01_18:23:18.209.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiahui Shi |
| SpeciesList | scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-03-01 18:23:11 | ID requested | |
| ⏵ 1 | 2023-03-01 18:23:18 | announced | |
| 2 | 2023-09-21 19:17:30 | announced | 2023-09-22: Add Subproject |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Mycolicibacterium smegmatis, arginine phosphorylation, proteomics |
Contact List
| Yao Zhang | |
|---|---|
| contact affiliation | National Center for Protein Sciences (Beijing) |
| contact email | zhangyaowsw@163.com |
| lab head | |
| Jiahui Shi | |
| contact affiliation | National Center for Protein Sciences (Beijing) |
| contact email | sjh1105211032@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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